Plasmonic nanobiosensor based on hairpin DNA for detection of trace oligonucleotides biomarker in cancers

Yanling Hu, Lei Zhang, Ying Zhang, Bin Wang, Yawei Wang, Quli Fan, Wei Huang, Lianhui Wang

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

MicroRNAs (miRNAs), a class of small, endogenous, noncoding RNA molecules, can serve as biomarkers for potential applications in cancer diagnosis, prognosis, and prediction due to its abnormal expression. As a result, a novel label-free biosensor with nanometer scale was prepared and employed in the detection of trace oligonucleotides based on the localized surface plasmon resonance (LSPR). The dielectric constant on the surface of DNA modified gold nanoparticle would change when probe single-strand DNA hybridized with target oligonucleotides, which resulted in the notable red shift of scattering peak position. The biosensor with excellent selectivity can be used in a real-time monitoring hybridization process. Notably, this method provided label-free detection of DNA and miRNA at single nanoparticle level with limit of detection up to 3 nM. Due to the advantages of LSPR scattering spectra, single nanoparticle biosensor can be designed for trace cancer-relevant miRNAs detection in the future.

Original languageEnglish
Pages (from-to)2459-2466
Number of pages8
JournalACS Applied Materials and Interfaces
Volume7
Issue number4
DOIs
StatePublished - 4 Feb 2015
Externally publishedYes

Keywords

  • biosensor
  • oligonucleotide detection
  • single gold nanoparticle
  • surface plasmon resonance

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