TY - JOUR
T1 - Plasmonic nanobiosensor based on hairpin DNA for detection of trace oligonucleotides biomarker in cancers
AU - Hu, Yanling
AU - Zhang, Lei
AU - Zhang, Ying
AU - Wang, Bin
AU - Wang, Yawei
AU - Fan, Quli
AU - Huang, Wei
AU - Wang, Lianhui
N1 - Publisher Copyright:
© 2014 American Chemical Society.
PY - 2015/2/4
Y1 - 2015/2/4
N2 - MicroRNAs (miRNAs), a class of small, endogenous, noncoding RNA molecules, can serve as biomarkers for potential applications in cancer diagnosis, prognosis, and prediction due to its abnormal expression. As a result, a novel label-free biosensor with nanometer scale was prepared and employed in the detection of trace oligonucleotides based on the localized surface plasmon resonance (LSPR). The dielectric constant on the surface of DNA modified gold nanoparticle would change when probe single-strand DNA hybridized with target oligonucleotides, which resulted in the notable red shift of scattering peak position. The biosensor with excellent selectivity can be used in a real-time monitoring hybridization process. Notably, this method provided label-free detection of DNA and miRNA at single nanoparticle level with limit of detection up to 3 nM. Due to the advantages of LSPR scattering spectra, single nanoparticle biosensor can be designed for trace cancer-relevant miRNAs detection in the future.
AB - MicroRNAs (miRNAs), a class of small, endogenous, noncoding RNA molecules, can serve as biomarkers for potential applications in cancer diagnosis, prognosis, and prediction due to its abnormal expression. As a result, a novel label-free biosensor with nanometer scale was prepared and employed in the detection of trace oligonucleotides based on the localized surface plasmon resonance (LSPR). The dielectric constant on the surface of DNA modified gold nanoparticle would change when probe single-strand DNA hybridized with target oligonucleotides, which resulted in the notable red shift of scattering peak position. The biosensor with excellent selectivity can be used in a real-time monitoring hybridization process. Notably, this method provided label-free detection of DNA and miRNA at single nanoparticle level with limit of detection up to 3 nM. Due to the advantages of LSPR scattering spectra, single nanoparticle biosensor can be designed for trace cancer-relevant miRNAs detection in the future.
KW - biosensor
KW - oligonucleotide detection
KW - single gold nanoparticle
KW - surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=84922458458&partnerID=8YFLogxK
U2 - 10.1021/am507218g
DO - 10.1021/am507218g
M3 - 文章
C2 - 25546579
AN - SCOPUS:84922458458
SN - 1944-8244
VL - 7
SP - 2459
EP - 2466
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 4
ER -