TY - JOUR
T1 - Optical/electrochemical methods for detecting mitochondrial energy metabolism
AU - Ji, Wenhui
AU - Tang, Xiao
AU - Du, Wei
AU - Lu, Yao
AU - Wang, Nanxiang
AU - Wu, Qiong
AU - Wei, Wei
AU - Liu, Jie
AU - Yu, Haidong
AU - Ma, Bo
AU - Li, Lin
AU - Huang, Wei
N1 - Publisher Copyright:
© 2021 The Royal Society of Chemistry.
PY - 2022
Y1 - 2022
N2 - This review highlights the biological importance of mitochondrial energy metabolism and the applications of multiple optical/electrochemical approaches to determine energy metabolites. Mitochondria, the main sites of oxidative phosphorylation and adenosine triphosphate (ATP) biosynthesis, provide the majority of energy required by aerobic cells for maintaining their physiological activity. They also participate in cell growth, differentiation, information transmission, and apoptosis. Multiple mitochondrial diseases, caused by internal or external factors, including oxidative stress, intense fluctuations of the ionic concentration, abnormal oxidative phosphorylation, changes in electron transport chain complex enzymes and mutations in mitochondrial DNA, can occur during mitochondrial energy metabolism. Therefore, developing accurate, sensitive, and specific methods for the in vivo and in vitro detection of mitochondrial energy metabolites is of great importance. In this review, we summarise the mitochondrial structure, functions, and crucial energy metabolic signalling pathways. The mechanism and applications of different optical/electrochemical methods are thoroughly reviewed. Finally, future research directions and challenges are proposed.
AB - This review highlights the biological importance of mitochondrial energy metabolism and the applications of multiple optical/electrochemical approaches to determine energy metabolites. Mitochondria, the main sites of oxidative phosphorylation and adenosine triphosphate (ATP) biosynthesis, provide the majority of energy required by aerobic cells for maintaining their physiological activity. They also participate in cell growth, differentiation, information transmission, and apoptosis. Multiple mitochondrial diseases, caused by internal or external factors, including oxidative stress, intense fluctuations of the ionic concentration, abnormal oxidative phosphorylation, changes in electron transport chain complex enzymes and mutations in mitochondrial DNA, can occur during mitochondrial energy metabolism. Therefore, developing accurate, sensitive, and specific methods for the in vivo and in vitro detection of mitochondrial energy metabolites is of great importance. In this review, we summarise the mitochondrial structure, functions, and crucial energy metabolic signalling pathways. The mechanism and applications of different optical/electrochemical methods are thoroughly reviewed. Finally, future research directions and challenges are proposed.
UR - http://www.scopus.com/inward/record.url?scp=85122850631&partnerID=8YFLogxK
U2 - 10.1039/d0cs01610a
DO - 10.1039/d0cs01610a
M3 - 文献综述
C2 - 34792041
AN - SCOPUS:85122850631
SN - 0306-0012
VL - 51
SP - 71
EP - 127
JO - Chemical Society Reviews
JF - Chemical Society Reviews
IS - 1
ER -