Optical/electrochemical methods for detecting mitochondrial energy metabolism

Wenhui Ji, Xiao Tang, Wei Du, Yao Lu, Nanxiang Wang, Qiong Wu, Wei Wei, Jie Liu, Haidong Yu, Bo Ma, Lin Li, Wei Huang

Research output: Contribution to journalReview articlepeer-review

68 Scopus citations

Abstract

This review highlights the biological importance of mitochondrial energy metabolism and the applications of multiple optical/electrochemical approaches to determine energy metabolites. Mitochondria, the main sites of oxidative phosphorylation and adenosine triphosphate (ATP) biosynthesis, provide the majority of energy required by aerobic cells for maintaining their physiological activity. They also participate in cell growth, differentiation, information transmission, and apoptosis. Multiple mitochondrial diseases, caused by internal or external factors, including oxidative stress, intense fluctuations of the ionic concentration, abnormal oxidative phosphorylation, changes in electron transport chain complex enzymes and mutations in mitochondrial DNA, can occur during mitochondrial energy metabolism. Therefore, developing accurate, sensitive, and specific methods for the in vivo and in vitro detection of mitochondrial energy metabolites is of great importance. In this review, we summarise the mitochondrial structure, functions, and crucial energy metabolic signalling pathways. The mechanism and applications of different optical/electrochemical methods are thoroughly reviewed. Finally, future research directions and challenges are proposed.

Original languageEnglish
Pages (from-to)71-127
Number of pages57
JournalChemical Society Reviews
Volume51
Issue number1
DOIs
StatePublished - 2022

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