One-micron resolution optical coherence tomography (OCT) in vivo for cellular level imaging

Dongyao Cui, Xinyu Liu, Jing Zhang, Xiaojun Yu, Sun Ding, Yuemei Luo, Jun Gu, Ping Shum, Linbo Liu

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

We developed a spectral domain OCT system combining two NIR, CW light sources of different spectral range. Its resolving power is validated by visualizing the cellular structures of zebra fish larvae in vivo. An NIR extended illumination from 755-1100 nm is achieved. The axial resolution is 1.27 μm in air, corresponding to 0.93 μm in tissue (n=1.36), which is the highest axial resolution using NIR, CW laser sources up to date to the best of our knowledge. In vivo imaging is conducted to demonstrate the resolving power of proposed one-micron resolution OCT system. The top and bottom surfaces of individual disk-like red blood cell is reliably visualized, as well as flat, spindle shaped endothelial cells lining along the luminal surface of the blood vessel wall. This study provides a viable solution for cellular and subcellular level OCT imaging system which is also very competitive in cost.

Original languageEnglish
Title of host publicationOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
EditorsValery V. Tuchin, James G. Fujimoto, Joseph A. Izatt
PublisherSPIE
ISBN (Electronic)9781628414028
DOIs
StatePublished - 2015
Externally publishedYes
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX - San Francisco, United States
Duration: 8 Feb 201511 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9312
ISSN (Print)1605-7422

Conference

ConferenceOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
Country/TerritoryUnited States
CitySan Francisco
Period8/02/1511/02/15

Keywords

  • In vivo
  • Optical coherence tomography (OCT)
  • Resolution

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