TY - JOUR
T1 - Morphology-Tunable Fluorescent Nanoparticles
T2 - Synthesis, Photophysical Properties and Two-Photon Cell Imaging
AU - Hu, Xiaoming
AU - Yin, Chao
AU - Hu, Wenbo
AU - Yang, Zhen
AU - Li, Jie
AU - Li, Xiang
AU - Lu, Xiaomei
AU - Zhao, Hui
AU - Tang, Yufu
AU - Fan, Quli
AU - Huang, Wei
N1 - Publisher Copyright:
Copyright © 2015 SIOC, CAS, Shanghai & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - A novel water-soluble fluorescent amphiphile based on amino polyethylene glycol (PEG-NH2) substituted oligo(p-phenyleneethynylene) (OPE) was designed and synthesized successfully. Taking anion OPE amphiphile as a comparison, the photophysical features were investigated through ultraviolet absorption (UV) and photoluminescence (PL) analyses. Due to the hydrophobic-hydrophilic property of the OPE conjugated molecule, self-assembled nanoparticles with the size ranging from 19.6 to 93.5 nm along with the change of morphology from grain to strawberry were conveniently prepared via adjusting concentrations of OPE aqueous solution. Interestingly, after aging for a period of time, homogeneous hollow nanospheres were spontaneously constructed with a diameter of about 200 nm. Cytotoxicity test and cellular uptake behavior of the nanoparticles were further investigated to evaluate their potential biomedical applications. Subsequently, the promising applications of two-photon cell imaging were explored using human pancreatic cancer cells (PANC-1 cells), which indicated that the nanoparticles were mainly located within the cell cytoplasm. Monodisperse single nanoparticles can be formed by self-assembly firstly, and then gathered to large aggregates with the increase of amphiphilic OPE concentration. Finally, these aggregates would crimp to form homogeneous hollow nanospheres through aging the solution for one week.
AB - A novel water-soluble fluorescent amphiphile based on amino polyethylene glycol (PEG-NH2) substituted oligo(p-phenyleneethynylene) (OPE) was designed and synthesized successfully. Taking anion OPE amphiphile as a comparison, the photophysical features were investigated through ultraviolet absorption (UV) and photoluminescence (PL) analyses. Due to the hydrophobic-hydrophilic property of the OPE conjugated molecule, self-assembled nanoparticles with the size ranging from 19.6 to 93.5 nm along with the change of morphology from grain to strawberry were conveniently prepared via adjusting concentrations of OPE aqueous solution. Interestingly, after aging for a period of time, homogeneous hollow nanospheres were spontaneously constructed with a diameter of about 200 nm. Cytotoxicity test and cellular uptake behavior of the nanoparticles were further investigated to evaluate their potential biomedical applications. Subsequently, the promising applications of two-photon cell imaging were explored using human pancreatic cancer cells (PANC-1 cells), which indicated that the nanoparticles were mainly located within the cell cytoplasm. Monodisperse single nanoparticles can be formed by self-assembly firstly, and then gathered to large aggregates with the increase of amphiphilic OPE concentration. Finally, these aggregates would crimp to form homogeneous hollow nanospheres through aging the solution for one week.
KW - hollow nanospheres
KW - oligo(p-phenyleneethynylene) amphiphile
KW - two-photon cell imaging
KW - water-soluble fluorescent nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84939650502&partnerID=8YFLogxK
U2 - 10.1002/cjoc.201500248
DO - 10.1002/cjoc.201500248
M3 - 文章
AN - SCOPUS:84939650502
SN - 1001-604X
VL - 33
SP - 888
EP - 896
JO - Chinese Journal of Chemistry
JF - Chinese Journal of Chemistry
IS - 8
ER -
