TY - JOUR
T1 - Metal coordination assisted thermo-sensitive magnetic imprinted microspheres for selective adsorption and efficient elution of proteins
AU - Zhou, Jingjing
AU - Wang, Mingqi
AU - Zhang, Baoliang
AU - Zhang, Qiuyu
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/3/5
Y1 - 2021/3/5
N2 - Protein recognition and separation with high efficiency, low cost and facile construction has been hampered by the deficient development of fabrication methods. Toward this issue, we proposed a functionally integrated strategy by combining metal coordination, thermo-sensitive building blocks and hollow Fe3O4@mSiO2 microspheres (mSiO2 referred as mesoporous silica) with molecularly imprinted polymers (MIPs), to realize selective adsorption and effective desorption of the targeted protein bovine serum albumin (BSA). Herein, a series of metal ions (Mn2+, Fe3+, Ni2+, Cu2+, Zn2+) were selected to chelate onto the surface of amino groups modified substrates to investigate the metal coordination effect, wherein Ni2+ exhibited the optimal BSA immobilization ability. Beneficial from the sufficient templates coordinated by Ni2+, our MSS@Ni2+-MIPs (represented Ni2+-involved imprinted microspheres) with well-designed composite structure showed the best rebinding performance (Q = 182.18 mg/g, IF = 4.78) and rapid adsorption equilibrium kinetics (30 min) at 32 °C. Although template proteins were seized by the strong metal coordination, we obtained the satisfactory elution efficiency (61.97 %) at 20 °C assisted with the swollen thermo-sensitive components. Based on this facile, smart and versatile imprinting approach, the well-defined materials would hold great promising in bio-applications.
AB - Protein recognition and separation with high efficiency, low cost and facile construction has been hampered by the deficient development of fabrication methods. Toward this issue, we proposed a functionally integrated strategy by combining metal coordination, thermo-sensitive building blocks and hollow Fe3O4@mSiO2 microspheres (mSiO2 referred as mesoporous silica) with molecularly imprinted polymers (MIPs), to realize selective adsorption and effective desorption of the targeted protein bovine serum albumin (BSA). Herein, a series of metal ions (Mn2+, Fe3+, Ni2+, Cu2+, Zn2+) were selected to chelate onto the surface of amino groups modified substrates to investigate the metal coordination effect, wherein Ni2+ exhibited the optimal BSA immobilization ability. Beneficial from the sufficient templates coordinated by Ni2+, our MSS@Ni2+-MIPs (represented Ni2+-involved imprinted microspheres) with well-designed composite structure showed the best rebinding performance (Q = 182.18 mg/g, IF = 4.78) and rapid adsorption equilibrium kinetics (30 min) at 32 °C. Although template proteins were seized by the strong metal coordination, we obtained the satisfactory elution efficiency (61.97 %) at 20 °C assisted with the swollen thermo-sensitive components. Based on this facile, smart and versatile imprinting approach, the well-defined materials would hold great promising in bio-applications.
KW - Efficient elution
KW - Hollow mesoporous magnetic substrates
KW - Metal coordination
KW - Molecularly imprinted polymers
KW - Thermo-sensitivity
UR - http://www.scopus.com/inward/record.url?scp=85098463323&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2020.125981
DO - 10.1016/j.colsurfa.2020.125981
M3 - 文章
AN - SCOPUS:85098463323
SN - 0927-7757
VL - 612
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
M1 - 125981
ER -