Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathway

Bin Zhang, Xianglin Li, Xiaojie Zhou, Chen Ge Lou, Shenghang Wang, Huanhuan Lv, Gejing Zhang, Yanwen Fang, Dachuan Yin, Peng Shang

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Osteocytes are the mechano-sensors of bones. Large gradient high-static magnetic fields (LG-HMFs) produce stable, high-precision, and non-attenuation mechanical forces. We discovered that magnetic forces opposite to gravity inhibited MLO-Y4 osteocyte proliferation and viability by inducing structural damage and apoptosis. In contrast, magnetic force loading in the same direction as that of gravity promoted the proliferation and inhibited apoptosis of MLO-Y4 osteocytes. Differentially expressed gene (DEG) analysis after magnetic force stimulation indicated that the ECM-integrin-CSK axis responded most significantly to mechanical signals. Wisp2 was the most significant DEG between the 12 T upward and downward groups, showing the highest correlation with the Wnt pathway according to the STRING protein interaction database. Explaining the cellular and molecular mechanisms by which mechanical stimuli influence bone remodeling is currently the focus of osteocyte-related research. Our findings provide insights into the effects of LG-HMFs on bone cells, which have further implications in clinical practice.

Original languageEnglish
Article number107365
JournaliScience
Volume26
Issue number8
DOIs
StatePublished - 18 Aug 2023

Keywords

  • Cellular physiology
  • Developmental biology
  • Molecular biology

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