TY - JOUR
T1 - Lysosome-Assisted Mitochondrial Targeting Nanoprobe Based on Dye-Modified Upconversion Nanophosphors for Ratiometric Imaging of Mitochondrial Hydrogen Sulfide
AU - Li, Xiang
AU - Zhao, Hui
AU - Ji, Yu
AU - Yin, Chao
AU - Li, Jie
AU - Yang, Zhen
AU - Tang, Yufu
AU - Zhang, Qichun
AU - Fan, Quli
AU - Huang, Wei
N1 - Publisher Copyright:
Copyright © 2018 American Chemical Society.
PY - 2018/11/21
Y1 - 2018/11/21
N2 - Hydrogen sulfide (H 2 S) is a versatile modulator in mitochondria and involved in numerous diseases caused by mitochondrial dysfunction. Therefore, many efforts have been made to develop fluorescent probes for mitochondrial H 2 S detection. However, these cationic small molecule probes are inapplicable for in vivo imaging because of the shallow tissue penetration and poor biostability. Herein, a ratiometric upconversion luminescence nanoprobe with an acid-activated targeting strategy is developed for detecting and bioimaging of mitochondrial H 2 S. The merocyanine triphenylamine-merocyanine (TPAMC)-modified upconversion nanophosphors, acting as the targeting and response component, are encapsulated into a pH-sensitive husk, composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(poly(ethylene glycol))-2000] (DSPE-PEG) and poly(l-histidine)-b-PEG, which improved the nanoprobe's stability during transport in vivo. Under lysosomal pH, the PEG shell is interrupted and the targeting sites are exposed to further attach to mitochondria. Taking advantage of the luminescence resonance energy transfer process between TPAMC and upconversion nanophosphors, the ratiometric detection of mitochondrial H 2 S can be achieved with high selectivity and sensitivity. Cellular testing reveals the precise targeting to mitochondria via a lysosome delivery process. Importantly, the nanoprobe can be used for monitoring mitochondrial H 2 S levels in living cells and colon cancer mouse models.
AB - Hydrogen sulfide (H 2 S) is a versatile modulator in mitochondria and involved in numerous diseases caused by mitochondrial dysfunction. Therefore, many efforts have been made to develop fluorescent probes for mitochondrial H 2 S detection. However, these cationic small molecule probes are inapplicable for in vivo imaging because of the shallow tissue penetration and poor biostability. Herein, a ratiometric upconversion luminescence nanoprobe with an acid-activated targeting strategy is developed for detecting and bioimaging of mitochondrial H 2 S. The merocyanine triphenylamine-merocyanine (TPAMC)-modified upconversion nanophosphors, acting as the targeting and response component, are encapsulated into a pH-sensitive husk, composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(poly(ethylene glycol))-2000] (DSPE-PEG) and poly(l-histidine)-b-PEG, which improved the nanoprobe's stability during transport in vivo. Under lysosomal pH, the PEG shell is interrupted and the targeting sites are exposed to further attach to mitochondria. Taking advantage of the luminescence resonance energy transfer process between TPAMC and upconversion nanophosphors, the ratiometric detection of mitochondrial H 2 S can be achieved with high selectivity and sensitivity. Cellular testing reveals the precise targeting to mitochondria via a lysosome delivery process. Importantly, the nanoprobe can be used for monitoring mitochondrial H 2 S levels in living cells and colon cancer mouse models.
KW - hydrogen sulfide
KW - mitochondria
KW - nanoprobe
KW - ratiometric
KW - upconversion luminescence
UR - http://www.scopus.com/inward/record.url?scp=85056924518&partnerID=8YFLogxK
U2 - 10.1021/acsami.8b16818
DO - 10.1021/acsami.8b16818
M3 - 文章
C2 - 30387597
AN - SCOPUS:85056924518
SN - 1944-8244
VL - 10
SP - 39544
EP - 39556
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 46
ER -