TY - JOUR
T1 - LAMP-on-a-chip
T2 - Revising microfluidic platforms for loop-mediated DNA amplification
AU - Zhang, Haoqing
AU - Xu, Ying
AU - Fohlerova, Zdenka
AU - Chang, Honglong
AU - Iliescu, Ciprian
AU - Neuzil, Pavel
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/4
Y1 - 2019/4
N2 - Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is simpler in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.
AB - Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is simpler in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.
KW - Digital LAMP
KW - LAMP
KW - Microfluidic
KW - Multi gene detection
KW - PCR
KW - POC
KW - Paper-based LAMP
KW - Single gene detection
UR - http://www.scopus.com/inward/record.url?scp=85061142460&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2019.01.015
DO - 10.1016/j.trac.2019.01.015
M3 - 文献综述
AN - SCOPUS:85061142460
SN - 0165-9936
VL - 113
SP - 44
EP - 53
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
ER -
