Immunomagnetic metal-organic frameworks based coupling-free and extraction-free sensitive detection of aflatoxin B₁ in peanut oils

Conventional AFB₁ detection methods are hindered by cumbersome pretreatment procedures, primarily due to the lack of new sample pretreatment materials and technologies. This work developed a novel coupling-free synthesis strategy for the immunomagnetic metal-organic frameworks (IMMOFs), focusing on its effective and promising application in the extraction-free detection of AFB₁. By coupling with UPLC-FLD, a sensitive quantification detection method for AFB₁ was developed, exhibiting a linear range of 0.5 to 20 μg/kg and a detection limit of 0.1 μg/kg. The spiked recoveries at three concentrations ranged from 90.3 % to 97.9 %, with RSDs of less than 6 %. Moreover, the established method was successfully employed for analyzing AFB₁ in naturally contaminated peanut oil samples, with results further confirmed by the traditional immunoaffinity column (IAC) method. This study provides an effective extraction-free detection technique for AFB₁ that addresses the limitations of the traditional methods.