High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

A. R. Qian; P. F. Yang; L. F. Hu; W. Zhang; S. M. Di; Z. Wang; J. Han; X. Gao; P. Shang

doi:10.1016/j.asr.2010.04.012

High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

A. R. Qian, P. F. Yang, L. F. Hu, W. Zhang, S. M. Di, Z. Wang, J. Han, X. Gao, P. Shang

School of life sciences

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture and genes associated with the cytoskeleton in osteoblasts (MC3T3-E1 and MG-63 cells) were investigated using confocal microscopy, real-time polymerase chain reaction (PCR) and atomic force microscopy (AFM). The findings showed that, under diamagnetic levitation conditions, the architecture and average height of the cytoskeleton and surface roughness in osteoblasts were dramatically altered. HMGE affects cytoskeleton arrangement and cytoskeleton-associated gene expression.

Original language	English
Pages (from-to)	687-700
Number of pages	14
Journal	Advances in Space Research
Volume	46
Issue number	6
DOIs	https://doi.org/10.1016/j.asr.2010.04.012
State	Published - 15 Sep 2010

Keywords

Cytoskeleton
Diamagnetic levitation
High magnetic gradient environment
Osteoblast
Simulated weightlessness

Access to Document

10.1016/j.asr.2010.04.012

Cite this

@article{748f59862f1c4c14bc13c2f803b44007,

title = "High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro",

abstract = "The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture and genes associated with the cytoskeleton in osteoblasts (MC3T3-E1 and MG-63 cells) were investigated using confocal microscopy, real-time polymerase chain reaction (PCR) and atomic force microscopy (AFM). The findings showed that, under diamagnetic levitation conditions, the architecture and average height of the cytoskeleton and surface roughness in osteoblasts were dramatically altered. HMGE affects cytoskeleton arrangement and cytoskeleton-associated gene expression.",

keywords = "Cytoskeleton, Diamagnetic levitation, High magnetic gradient environment, Osteoblast, Simulated weightlessness",

author = "Qian, {A. R.} and Yang, {P. F.} and Hu, {L. F.} and W. Zhang and Di, {S. M.} and Z. Wang and J. Han and X. Gao and P. Shang",

year = "2010",

month = sep,

day = "15",

doi = "10.1016/j.asr.2010.04.012",

language = "英语",

volume = "46",

pages = "687--700",

journal = "Advances in Space Research",

issn = "0273-1177",

publisher = "Elsevier Ltd",

number = "6",

}

TY - JOUR

T1 - High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

AU - Qian, A. R.

AU - Yang, P. F.

AU - Hu, L. F.

AU - Zhang, W.

AU - Di, S. M.

AU - Wang, Z.

AU - Han, J.

AU - Gao, X.

AU - Shang, P.

PY - 2010/9/15

Y1 - 2010/9/15

N2 - The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture and genes associated with the cytoskeleton in osteoblasts (MC3T3-E1 and MG-63 cells) were investigated using confocal microscopy, real-time polymerase chain reaction (PCR) and atomic force microscopy (AFM). The findings showed that, under diamagnetic levitation conditions, the architecture and average height of the cytoskeleton and surface roughness in osteoblasts were dramatically altered. HMGE affects cytoskeleton arrangement and cytoskeleton-associated gene expression.

AB - The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture and genes associated with the cytoskeleton in osteoblasts (MC3T3-E1 and MG-63 cells) were investigated using confocal microscopy, real-time polymerase chain reaction (PCR) and atomic force microscopy (AFM). The findings showed that, under diamagnetic levitation conditions, the architecture and average height of the cytoskeleton and surface roughness in osteoblasts were dramatically altered. HMGE affects cytoskeleton arrangement and cytoskeleton-associated gene expression.

KW - Cytoskeleton

KW - Diamagnetic levitation

KW - High magnetic gradient environment

KW - Osteoblast

KW - Simulated weightlessness

UR - http://www.scopus.com/inward/record.url?scp=77955553369&partnerID=8YFLogxK

U2 - 10.1016/j.asr.2010.04.012

DO - 10.1016/j.asr.2010.04.012

M3 - 文章

AN - SCOPUS:77955553369

SN - 0273-1177

VL - 46

SP - 687

EP - 700

JO - Advances in Space Research

JF - Advances in Space Research

IS - 6

ER -

High magnetic gradient environment causes alterations of cytoskeleton and cytoskeleton-associated genes in human osteoblasts cultured in vitro

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this