Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

Liulin Wang; Buxiang Chen; Pingping Peng; Wenbo Hu; Zhipeng Liu; Xiaohua Pei; Weihong Zhao; Chengwu Zhang; Lin Li; Wei Huang

doi:10.1016/j.cclet.2017.07.016

Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

Liulin Wang, Buxiang Chen, Pingping Peng, Wenbo Hu, Zhipeng Liu, Xiaohua Pei, Weihong Zhao, Chengwu Zhang, Lin Li, Wei Huang

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, M_Cu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10⁻⁷ mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated M_Cu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

Original language	English
Pages (from-to)	1965-1968
Number of pages	4
Journal	Chinese Chemical Letters
Volume	28
Issue number	10
DOIs	https://doi.org/10.1016/j.cclet.2017.07.016
State	Published - Oct 2017
Externally published	Yes

Keywords

Copper(II)
Fluorescence imaging
Fluorogenic probe
Mitochondria
Photocontrollable

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10.1016/j.cclet.2017.07.016

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title = "Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells",

abstract = "Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.",

keywords = "Copper(II), Fluorescence imaging, Fluorogenic probe, Mitochondria, Photocontrollable",

author = "Liulin Wang and Buxiang Chen and Pingping Peng and Wenbo Hu and Zhipeng Liu and Xiaohua Pei and Weihong Zhao and Chengwu Zhang and Lin Li and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2017 The Author",

year = "2017",

month = oct,

doi = "10.1016/j.cclet.2017.07.016",

language = "英语",

volume = "28",

pages = "1965--1968",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

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T1 - Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

AU - Wang, Liulin

AU - Chen, Buxiang

AU - Peng, Pingping

AU - Hu, Wenbo

AU - Liu, Zhipeng

AU - Pei, Xiaohua

AU - Zhao, Weihong

AU - Zhang, Chengwu

AU - Li, Lin

AU - Huang, Wei

PY - 2017/10

Y1 - 2017/10

N2 - Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

AB - Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

KW - Copper(II)

KW - Fluorescence imaging

KW - Fluorogenic probe

KW - Mitochondria

KW - Photocontrollable

UR - http://www.scopus.com/inward/record.url?scp=85026763732&partnerID=8YFLogxK

U2 - 10.1016/j.cclet.2017.07.016

DO - 10.1016/j.cclet.2017.07.016

M3 - 文章

AN - SCOPUS:85026763732

SN - 1001-8417

VL - 28

SP - 1965

EP - 1968

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 10

ER -

Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

Abstract

Keywords

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