TY - JOUR
T1 - Extraction and sequencing of trace DNA from Cornu cervi pantotrichum
AU - Wang, Jianyun
AU - He, Guangxin
AU - Fu, Wen
AU - Su, Bing
AU - Wang, Wen
AU - Zhang, Yaping
AU - Lu, Yuan
AU - He, Mu
AU - Zhu, Zhaoyun
PY - 1997
Y1 - 1997
N2 - The trace DNA extraction method was used to extract DNA from the blood, hair, Cornu cervi pantotrichum. hairy antler of the sika deer, Cervus nippon, and penis of Bulbalus bublis and Asinus vulgaris. A 307bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions. The purified PCR products were sequenced by the dideoxy method. DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer, but the sequence of the so - called hairy antler is quite different, implying that it is not from the sika deer. In addition the phylogenetic relationships among different species are congruent with the traditional known phylogeny.
AB - The trace DNA extraction method was used to extract DNA from the blood, hair, Cornu cervi pantotrichum. hairy antler of the sika deer, Cervus nippon, and penis of Bulbalus bublis and Asinus vulgaris. A 307bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions. The purified PCR products were sequenced by the dideoxy method. DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer, but the sequence of the so - called hairy antler is quite different, implying that it is not from the sika deer. In addition the phylogenetic relationships among different species are congruent with the traditional known phylogeny.
KW - Animal medicine appraisal
KW - Cornu cervi pantotrichum
KW - DNA sequence analysis identification
UR - http://www.scopus.com/inward/record.url?scp=0031240336&partnerID=8YFLogxK
M3 - 文章
C2 - 11038922
AN - SCOPUS:0031240336
SN - 1001-5302
VL - 22
SP - 579
EP - 583
JO - Zhongguo Zhongyao Zazhi
JF - Zhongguo Zhongyao Zazhi
IS - 10
ER -