Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

A. Wei; X. W. Sun; J. X. Wang; Y. Lei; X. P. Cai; C. M. Li; Z. L. Dong; W. Huang

doi:10.1063/1.2356307

Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

A. Wei, X. W. Sun, J. X. Wang, Y. Lei, X. P. Cai, C. M. Li, Z. L. Dong, W. Huang

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Abstract

We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm^-2 mM^-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

Original language	English
Article number	123902
Journal	Applied Physics Letters
Volume	89
Issue number	12
DOIs	https://doi.org/10.1063/1.2356307
State	Published - 2006
Externally published	Yes

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title = "Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition",

abstract = "We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.",

author = "A. Wei and Sun, {X. W.} and Wang, {J. X.} and Y. Lei and Cai, {X. P.} and Li, {C. M.} and Dong, {Z. L.} and W. Huang",

year = "2006",

doi = "10.1063/1.2356307",

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volume = "89",

journal = "Applied Physics Letters",

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TY - JOUR

T1 - Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

AU - Wei, A.

AU - Sun, X. W.

AU - Wang, J. X.

AU - Lei, Y.

AU - Cai, X. P.

AU - Li, C. M.

AU - Dong, Z. L.

AU - Huang, W.

PY - 2006

Y1 - 2006

N2 - We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

AB - We report herein a glucose biosensor based on glucose oxidase (GOx) immobilized on ZnO nanorod array grown by hydrothermal decomposition. In a phosphate buffer solution with a pH value of 7.4, negatively charged GOx was immobilized on positively charged ZnO nanorods through electrostatic interaction. At an applied potential of +0.8 V versus Ag/AgCl reference electrode, ZnO nanorods based biosensor presented a high and reproducible sensitivity of 23.1 μA cm-2 mM-1 with a response time of less than 5 s. The biosensor shows a linear range from 0.01 to 3.45 mM and an experiment limit of detection of 0.01 mM. An apparent Michaelis-Menten constant of 2.9 mM shows a high affinity between glucose and GOx immobilized on ZnO nanorods.

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U2 - 10.1063/1.2356307

DO - 10.1063/1.2356307

M3 - 文章

AN - SCOPUS:33748986409

SN - 0003-6951

VL - 89

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 12

M1 - 123902

ER -

Enzymatic glucose biosensor based on ZnO nanorod array grown by hydrothermal decomposition

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