TY - JOUR
T1 - Enhanced resonance light scattering based on biocatalytic growth of gold nanoparticles for biosensors design
AU - Shang, Li
AU - Chen, Hongjun
AU - Deng, Liu
AU - Dong, Shaojun
PY - 2008/2/28
Y1 - 2008/2/28
N2 - The biocatalytic growth of gold nanoparticles (Au-NPs) has been employed in the design of new optical biosensors based on the enhanced resonance light scattering (RLS) signals. Both absorption spectroscopy and transmission electron microscopy (TEM) analysis revealed Au-NP seeds could be effectively enlarged upon the reaction with H2O2, an important metabolite that could be generated by many biocatalytic reactions. Upon the stepwise enlargement of Au-NPs, the light scattering intensity could be greatly enhanced, which then allowed the quantitative detection of the analyte, H2O2. Further combination of the biocatalytic reaction that can yield H2O2 by using the enzyme, glucose oxidase, with the enlargement of Au-NPs enabled the design of a sensitive glucose biosensor using the RLS technique. In the present study, we could achieve the detection of glucose in a linear range of 1.0 × 10-6 M to 1.1 × 10-4 M, with detection limit of 6.8 × 10-7 M.
AB - The biocatalytic growth of gold nanoparticles (Au-NPs) has been employed in the design of new optical biosensors based on the enhanced resonance light scattering (RLS) signals. Both absorption spectroscopy and transmission electron microscopy (TEM) analysis revealed Au-NP seeds could be effectively enlarged upon the reaction with H2O2, an important metabolite that could be generated by many biocatalytic reactions. Upon the stepwise enlargement of Au-NPs, the light scattering intensity could be greatly enhanced, which then allowed the quantitative detection of the analyte, H2O2. Further combination of the biocatalytic reaction that can yield H2O2 by using the enzyme, glucose oxidase, with the enlargement of Au-NPs enabled the design of a sensitive glucose biosensor using the RLS technique. In the present study, we could achieve the detection of glucose in a linear range of 1.0 × 10-6 M to 1.1 × 10-4 M, with detection limit of 6.8 × 10-7 M.
KW - Biocatalytic growth
KW - Glucose
KW - Gold nanoparticles
KW - HO
KW - Resonance light scattering
UR - http://www.scopus.com/inward/record.url?scp=39049099099&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2007.10.024
DO - 10.1016/j.bios.2007.10.024
M3 - 文章
C2 - 18068347
AN - SCOPUS:39049099099
SN - 0956-5663
VL - 23
SP - 1180
EP - 1184
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 7
ER -