TY - JOUR
T1 - Effects of polysaccharide from mycelia of Ganoderma lucidum on intestinal barrier functions of rats
AU - Jin, Mingliang
AU - Zhu, Yimin
AU - Shao, Dongyan
AU - Zhao, Ke
AU - Xu, Chunlan
AU - Li, Qi
AU - Yang, Hui
AU - Huang, Qingsheng
AU - Shi, Junling
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The intestinal mucosal barriers play essential roles not only in the digestion and absorption of nutrients, but also the innate defense against most intestinal pathogens. In the present study, polysaccharide from the mycelia of Ganoderma lucidum was given via oral administration to rats (100 mg/kg body weight, 21 days) to investigate its effects on intestinal barrier functions, including the mechanical barrier, immunological barrier and biological barrier function. It was found that the polysaccharide administration could significantly up-regulate the expression of occludin, nuclear factor-κB p65 (NF-κB p65) and secretory immunoglobulin A (SIgA) in ileum, markedly improve the levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), and IL-4, and decrease the level of diamine oxidase (DAO) in serum. Meanwhile, rats from the polysaccharide group showed significant higher microbiota richness in cecum as reflected by the Chao 1 index compared with the control group. Moreover, the polysaccharide decreased the Firmicutes-to-Bacteroidetes ratio. Our results indicated that the polysaccharide from the mycelia of G. lucidum might be used as functional agent to regulate the intestinal barrier functions.
AB - The intestinal mucosal barriers play essential roles not only in the digestion and absorption of nutrients, but also the innate defense against most intestinal pathogens. In the present study, polysaccharide from the mycelia of Ganoderma lucidum was given via oral administration to rats (100 mg/kg body weight, 21 days) to investigate its effects on intestinal barrier functions, including the mechanical barrier, immunological barrier and biological barrier function. It was found that the polysaccharide administration could significantly up-regulate the expression of occludin, nuclear factor-κB p65 (NF-κB p65) and secretory immunoglobulin A (SIgA) in ileum, markedly improve the levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), and IL-4, and decrease the level of diamine oxidase (DAO) in serum. Meanwhile, rats from the polysaccharide group showed significant higher microbiota richness in cecum as reflected by the Chao 1 index compared with the control group. Moreover, the polysaccharide decreased the Firmicutes-to-Bacteroidetes ratio. Our results indicated that the polysaccharide from the mycelia of G. lucidum might be used as functional agent to regulate the intestinal barrier functions.
KW - Ganoderma lucidum
KW - Intestinal barrier function
KW - Polysaccharide
UR - http://www.scopus.com/inward/record.url?scp=84989822833&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2016.09.099
DO - 10.1016/j.ijbiomac.2016.09.099
M3 - 文章
C2 - 27693834
AN - SCOPUS:84989822833
SN - 0141-8130
VL - 94
SP - 1
EP - 9
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -