TY - JOUR
T1 - Effect of sustained release of recombinant rat insulin-like growth factor-1 from poly (lactide-CO-glycolide) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes
AU - Yu, Miao
AU - Hui, Xin Min
AU - Wang, Feng
AU - Zhang, Ning
AU - Zhang, He Peng
AU - Song, Ying Liang
PY - 2010/6
Y1 - 2010/6
N2 - Objective: To evaluate the effect of sustained release of recombinant rat insulin-like growth factor-1 (rrIGF-1) from poly (lactide-CO-glycolide) (PLGA) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes. Methods: Type 2 diabetes models were successfully established in 20 male Goto-Kakizaki rats, which were then randomly divided into treatment group (sustained release of rrIGF-1 from PLGA microspheres were loaded on the peri-implant areas, n=10) and diabetic group (loaded with isodose placebo from PLGA microspheres, n=10). Another ten male SD rats served as control group (did not sustain any loading). Titanium implants were inserted into the tibias of 30 diabetic and normal animals. Four, 5, and 8 weeks after implantation, local blood samples around the implants were obtained for the determination of serum osteocalcin (OCN), serum bone specific alkaline phosphatase (B-ALP), and serum procollagen I carboxyterminal propeptide (PICP) with enzyme linked immunosorbent assays. Results: Four weeks after implantation, OCN, B-ALP, and PICP were significantly lower in both treatment group and diabetic group than in control group (both P < 0.05). Five weeks after implantation, serum OCN and B-ALP levels of the diabetic group were significantly lower than those of the other two groups (all P < 0.05). Serum PICP levels of both diabetic group and treatment group were significantly lower than that of control group (both P < 0.05). The OCN level in the treatment group was significantly higher in the post-operative 5th week than in the post-operative 4th week, while the PICP levels in the diabetic group were significantly lower than those in the treatment group and control group in the post-operative 8th week (both P < 0.05). Conclusion: Sustained release of rrIGF-1 from PLGA microspheres loaded on the local peri-implant areas can promote bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes.
AB - Objective: To evaluate the effect of sustained release of recombinant rat insulin-like growth factor-1 (rrIGF-1) from poly (lactide-CO-glycolide) (PLGA) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes. Methods: Type 2 diabetes models were successfully established in 20 male Goto-Kakizaki rats, which were then randomly divided into treatment group (sustained release of rrIGF-1 from PLGA microspheres were loaded on the peri-implant areas, n=10) and diabetic group (loaded with isodose placebo from PLGA microspheres, n=10). Another ten male SD rats served as control group (did not sustain any loading). Titanium implants were inserted into the tibias of 30 diabetic and normal animals. Four, 5, and 8 weeks after implantation, local blood samples around the implants were obtained for the determination of serum osteocalcin (OCN), serum bone specific alkaline phosphatase (B-ALP), and serum procollagen I carboxyterminal propeptide (PICP) with enzyme linked immunosorbent assays. Results: Four weeks after implantation, OCN, B-ALP, and PICP were significantly lower in both treatment group and diabetic group than in control group (both P < 0.05). Five weeks after implantation, serum OCN and B-ALP levels of the diabetic group were significantly lower than those of the other two groups (all P < 0.05). Serum PICP levels of both diabetic group and treatment group were significantly lower than that of control group (both P < 0.05). The OCN level in the treatment group was significantly higher in the post-operative 5th week than in the post-operative 4th week, while the PICP levels in the diabetic group were significantly lower than those in the treatment group and control group in the post-operative 8th week (both P < 0.05). Conclusion: Sustained release of rrIGF-1 from PLGA microspheres loaded on the local peri-implant areas can promote bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes.
KW - Bone specific alkaline phosphatase
KW - Goto-Kakizaki rats
KW - Osteocalcin
KW - Poly (lactide-CO-glycolide)
KW - Procollagen I carboxyterminal propeptide
KW - Recombinant rat insulin like growth factor-1
KW - Type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=77954727238&partnerID=8YFLogxK
U2 - 10.3881/j.issn.1000-503X.2010.03.004
DO - 10.3881/j.issn.1000-503X.2010.03.004
M3 - 文章
C2 - 20602874
AN - SCOPUS:77954727238
SN - 1000-503X
VL - 32
SP - 260
EP - 264
JO - Acta Academiae Medicinae Sinicae
JF - Acta Academiae Medicinae Sinicae
IS - 3
ER -
