TY - JOUR
T1 - Effect of high static magnetic fields on biological activities and iron metabolism in mlo-y4 osteocyte-like cells
AU - Yang, Jiancheng
AU - Zhang, Gejing
AU - Li, Qingmei
AU - Tang, Qinghua
AU - Feng, Yan
AU - Shang, Peng
AU - Zeng, Yuhong
N1 - Publisher Copyright:
© 2021 by the authors. Li-censee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein ex-pression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were stud-ied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure.
AB - There are numerous studies that investigate the effects of static magnetic fields (SMFs) on osteoblasts and osteoclasts. However, although osteocytes are the most abundant cell type in bone tissue, there are few studies on the biological effects of osteocytes under magnetic fields. Iron is a necessary microelement that is involved in numerous life activities in cells. Studies have shown that high static magnetic fields (HiSMF) can regulate cellular iron metabolism. To illustrate the effect of HiSMF on activities of osteocytes, and whether iron is involved in this process, HiSMF of 16 tesla (T) was used, and the changes in cellular morphology, cytoskeleton, function-related protein ex-pression, secretion of various cytokines, and iron metabolism in osteocytes under HiSMF were stud-ied. In addition, the biological effects of HiSMF combined with iron preparation and iron chelator on osteocytes were also investigated. The results showed that HiSMF promoted cellular viability, decreased apoptosis, increased the fractal dimension of the cytoskeleton, altered the secretion of cytokines, and increased iron levels in osteocytes. Moreover, it was found that the biological effects of osteocytes under HiSMF are attenuated or enhanced by treatment with a certain concentration of iron. These data suggest that HiSMF-regulated cellular iron metabolism may be involved in altering the biological effects of osteocytes under HiSMF exposure.
KW - Apoptosis
KW - Cell viability
KW - Cytoskeleton
KW - High static magnetic fields
KW - Iron metabolism
KW - Osteocytes
UR - http://www.scopus.com/inward/record.url?scp=85120941261&partnerID=8YFLogxK
U2 - 10.3390/cells10123519
DO - 10.3390/cells10123519
M3 - 文章
C2 - 34944025
AN - SCOPUS:85120941261
SN - 2073-4409
VL - 10
JO - Cells
JF - Cells
IS - 12
M1 - 3519
ER -