TY - JOUR
T1 - Design of flexible dendrimer-grafted flower-like magnetic microcarriers for penicillin G acylase immobilization
AU - Li, Xue
AU - Tian, Lei
AU - Ali, Zafar
AU - Wang, Wenyan
AU - Zhang, Qiuyu
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.
AB - Micron-sized flower-like porous Fe3O4 microparticles were fabricated by improved solvothermal method. Then, flexible dendrimer-grafted Fe3O4@SiO2–NH2/PAMAM microcarriers were obtained by functionalized modification of Michael addition and amidation process. The immobilization of penicillin G acylase on the carrier was realized by cross-linking of glutaraldehyde, and we investigated the optimum immobilization conditions. Under the optimal immobilized conditions, the reaction activity could reach 3098.96 U/g. When the reaction temperature was 47 °C, the immobilized PGA had the highest activity of 394.74 U/g, which indicated that the immobilized PGA had better thermal stability than free PGA. The activity of immobilized enzyme maintained 82.14% after reused for nine times, which indicated that the immobilized enzyme had good stability and good industrial application potential.
UR - http://www.scopus.com/inward/record.url?scp=85031906231&partnerID=8YFLogxK
U2 - 10.1007/s10853-017-1581-9
DO - 10.1007/s10853-017-1581-9
M3 - 文章
AN - SCOPUS:85031906231
SN - 0022-2461
VL - 53
SP - 937
EP - 947
JO - Journal of Materials Science
JF - Journal of Materials Science
IS - 2
ER -