Dense Packed Drivable Optrode Array for Precise Optical Stimulation and Neural Recording in Multiple-Brain Regions

Longchun Wang, Chaofan Ge, Fang Wang, Zhejun Guo, Wen Hong, Chunpeng Jiang, Bowen Ji, Minghao Wang, Chengyu Li, Bomin Sun, Jingquan Liu

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The input-output function of neural networks is complicated due to the huge number of neurons and synapses, and some high-density implantable electrophysiology recording tools with a plane structure have been developed for neural circuit studies in recent years. However, traditional plane probes are limited by the record-only function and inability to monitor multiple-brain regions simultaneously, and the complete cognition of neural networks still has a long way away. Herein, we develop a three-dimensional (3D) high-density drivable optrode array for multiple-brain recording and precise optical stimulation simultaneously. The optrode array contains four-layer probes with 1024 microelectrodes and two thinned optical fibers assembled into a 3D-printed drivable module. The recording performance of microelectrodes is optimized by electrochemical modification, and precise implantation depth control of drivable optrodes is verified in agar. Moreover, in vivo experiments indicate neural activities from CA1 and dentate gyrus regions are monitored, and a tracking of the neuron firing for 2 weeks is achieved. The suppression of neuron firing by blue light has been realized through high-density optrodes during optogenetics experiments. With the feature of large-scale recording, optoelectronic integration, and 3D assembly, the high-density drivable optrode array possesses an important value in the research of brain diseases and neural networks.

Original languageEnglish
Pages (from-to)4126-4135
Number of pages10
JournalACS Sensors
Volume6
Issue number11
DOIs
StatePublished - 26 Nov 2021

Keywords

  • drivable
  • electrophysiology recording
  • high density
  • optogenetics
  • probe array

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