TY - JOUR
T1 - Corrigendum to “Nitrite-dependent anaerobic oxidation decreases methane emissions from peatlands” [Soil Biology and Biochemistry 169, 108658] (Soil Biology and Biochemistry (2022) 169, (S0038071722001158), (10.1016/j.soilbio.2022.108658))
AU - Shi, Yao
AU - Ma, Qingxu
AU - Kuzyakov, Yakov
AU - Sheng, Lianxi
AU - Liu, Hanyu
AU - Wang, Zhongqiang
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/3
Y1 - 2023/3
N2 - We acknowledge that the description of the 13CH4 addition in the 2.5 Methylomirabilis activity measurements may be ambiguous in the original paper titled “Nitrite-dependent anaerobic oxidation decreases methane emissions from peatlands” (Shi et al., 2022). Therefore, we provide here the detailed calculation protocols for Methylomirabilis copy number and nitrite-dependent anaerobic methane oxidation (n-damo) rate. • 2.5 Methylomirabilis activity measurementsA corrected sentence should be taken as follows: “0.1 mL of NaNO2 was injected into all vials, resulting in a concentration of 100 μmol N L−1 in the soil-water mixtures, and then 2 mL of 13CH4 (99.9% 13C atom-%) was injected into all vials with a syringe the vials were placed flat and another needle was inserted to drain the solution. Therefore, for the headspace issue, 2 mL of 13CH4 replaces 2 mL of the solution, that is, the headspace is occupied by 2 mL of 13CH4, at which point the volume fraction of 13CH4 in the vials was 2/12 mL equals 16.7% (v/v). • Calculation of the Methylomirabilis copy number 1. Quantitative plasmid information Plasmid M13 ± sequencingThe constructed plasmids were identified by sequencing without errors and the values of plasmid OD260 were measured by UV spectrophotometer and converted to copy number (copies μl−1) by the following equations: [Formula presented] [Formula presented] [Formula presented] 2. Preparation of standard curve samplesBased on 10-fold gradient dilution of each plasmid constructed, 90 μl dilution +10 μl plasmid, generating series based on 4–6 concentrations range prescribed by pre-experiments. • Calculation of n-damo rateThe n-damo determination and calculation methods were derived from Ettwig et al. (2009) and Hu et al. (2014). The detailed n-damo rate was calculated as follows: For isotopic analysis, the δ13C PDB values were converted to the percentage of 13C atom (13C AT %) using the equation: [Formula presented] [Formula presented] The n-damo rates were calculated as follows: [Formula presented] where Rn-damo (μmol g−1 h−1) is the measured n-damo rate Rstandard is 0.0112372 V is 2 mL Vm is 22.4 L mol−1 DW (g) is the dry weight of the peat samples T (h) is the incubation timeThese corrections neither impact the results nor the discussion of the study, and the conclusions likewise prevail. The authors apologize for any inconvenience.
AB - We acknowledge that the description of the 13CH4 addition in the 2.5 Methylomirabilis activity measurements may be ambiguous in the original paper titled “Nitrite-dependent anaerobic oxidation decreases methane emissions from peatlands” (Shi et al., 2022). Therefore, we provide here the detailed calculation protocols for Methylomirabilis copy number and nitrite-dependent anaerobic methane oxidation (n-damo) rate. • 2.5 Methylomirabilis activity measurementsA corrected sentence should be taken as follows: “0.1 mL of NaNO2 was injected into all vials, resulting in a concentration of 100 μmol N L−1 in the soil-water mixtures, and then 2 mL of 13CH4 (99.9% 13C atom-%) was injected into all vials with a syringe the vials were placed flat and another needle was inserted to drain the solution. Therefore, for the headspace issue, 2 mL of 13CH4 replaces 2 mL of the solution, that is, the headspace is occupied by 2 mL of 13CH4, at which point the volume fraction of 13CH4 in the vials was 2/12 mL equals 16.7% (v/v). • Calculation of the Methylomirabilis copy number 1. Quantitative plasmid information Plasmid M13 ± sequencingThe constructed plasmids were identified by sequencing without errors and the values of plasmid OD260 were measured by UV spectrophotometer and converted to copy number (copies μl−1) by the following equations: [Formula presented] [Formula presented] [Formula presented] 2. Preparation of standard curve samplesBased on 10-fold gradient dilution of each plasmid constructed, 90 μl dilution +10 μl plasmid, generating series based on 4–6 concentrations range prescribed by pre-experiments. • Calculation of n-damo rateThe n-damo determination and calculation methods were derived from Ettwig et al. (2009) and Hu et al. (2014). The detailed n-damo rate was calculated as follows: For isotopic analysis, the δ13C PDB values were converted to the percentage of 13C atom (13C AT %) using the equation: [Formula presented] [Formula presented] The n-damo rates were calculated as follows: [Formula presented] where Rn-damo (μmol g−1 h−1) is the measured n-damo rate Rstandard is 0.0112372 V is 2 mL Vm is 22.4 L mol−1 DW (g) is the dry weight of the peat samples T (h) is the incubation timeThese corrections neither impact the results nor the discussion of the study, and the conclusions likewise prevail. The authors apologize for any inconvenience.
UR - http://www.scopus.com/inward/record.url?scp=85146456098&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2022.108915
DO - 10.1016/j.soilbio.2022.108915
M3 - 评论/辩论
AN - SCOPUS:85146456098
SN - 0038-0717
VL - 178
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
M1 - 108915
ER -