Corrigendum to “Nitrite-dependent anaerobic oxidation decreases methane emissions from peatlands” [Soil Biology and Biochemistry 169, 108658] (Soil Biology and Biochemistry (2022) 169, (S0038071722001158), (10.1016/j.soilbio.2022.108658))

Yao Shi, Qingxu Ma, Yakov Kuzyakov, Lianxi Sheng, Hanyu Liu, Zhongqiang Wang

Research output: Contribution to journalComment/debate

Abstract

We acknowledge that the description of the 13CH4 addition in the 2.5 Methylomirabilis activity measurements may be ambiguous in the original paper titled “Nitrite-dependent anaerobic oxidation decreases methane emissions from peatlands” (Shi et al., 2022). Therefore, we provide here the detailed calculation protocols for Methylomirabilis copy number and nitrite-dependent anaerobic methane oxidation (n-damo) rate. • 2.5 Methylomirabilis activity measurementsA corrected sentence should be taken as follows: “0.1 mL of NaNO2 was injected into all vials, resulting in a concentration of 100 μmol N L−1 in the soil-water mixtures, and then 2 mL of 13CH4 (99.9% 13C atom-%) was injected into all vials with a syringe the vials were placed flat and another needle was inserted to drain the solution. Therefore, for the headspace issue, 2 mL of 13CH4 replaces 2 mL of the solution, that is, the headspace is occupied by 2 mL of 13CH4, at which point the volume fraction of 13CH4 in the vials was 2/12 mL equals 16.7% (v/v). • Calculation of the Methylomirabilis copy number 1. Quantitative plasmid information Plasmid M13 ± sequencingThe constructed plasmids were identified by sequencing without errors and the values of plasmid OD260 were measured by UV spectrophotometer and converted to copy number (copies μl−1) by the following equations: [Formula presented] [Formula presented] [Formula presented] 2. Preparation of standard curve samplesBased on 10-fold gradient dilution of each plasmid constructed, 90 μl dilution +10 μl plasmid, generating series based on 4–6 concentrations range prescribed by pre-experiments. • Calculation of n-damo rateThe n-damo determination and calculation methods were derived from Ettwig et al. (2009) and Hu et al. (2014). The detailed n-damo rate was calculated as follows: For isotopic analysis, the δ13C PDB values were converted to the percentage of 13C atom (13C AT %) using the equation: [Formula presented] [Formula presented] The n-damo rates were calculated as follows: [Formula presented] where Rn-damo (μmol g−1 h−1) is the measured n-damo rate Rstandard is 0.0112372 V is 2 mL Vm is 22.4 L mol−1 DW (g) is the dry weight of the peat samples T (h) is the incubation timeThese corrections neither impact the results nor the discussion of the study, and the conclusions likewise prevail. The authors apologize for any inconvenience.

Original languageEnglish
Article number108915
JournalSoil Biology and Biochemistry
Volume178
DOIs
StatePublished - Mar 2023
Externally publishedYes

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