TY - JOUR
T1 - Controllable and Scaffold-Free Formation of 3D Multicellular Architectures Using a Bipolar Electrode Array
AU - Wu, Yupan
AU - Zhang, Haohao
AU - Yue, Yuanbo
AU - Wang, Shaoxi
AU - Meng, Yingqi
N1 - Publisher Copyright:
© 2024 Wiley-VCH GmbH.
PY - 2024/3/18
Y1 - 2024/3/18
N2 - The natural environment of cells in vivo is a 3D network in which cellular responses are determined by influences from surrounding cells on all sides. This complex system is hard to be recapitulated in a conventional way. It is highly desirable to replace 2D models with 3D cell culture models. Microfluidic technology offers a variety of advantageous approaches for the long-term 3D cell culture. Herein, a new cell patterning approach for generating cell clusters of specified size and shape by exploiting induced charge electroosmosis (ICEO) flow at a bipolar electrode array is exploited. Cell or particle clusters can be produced at the edges or centers of bipolar electrodes (BPEs) with controlled organization, by designing electrodes with arbitrary shapes. This platform is further extended to flexibly organize biological units with different properties via the ICEO or dielectrophoresis (DEP) based assembly. Heterotypic cell clusters can be produced at the bipolar electrode (BPE) by adjusting the applied frequencies and the sample injection sequence. This hybrid approach by integrating ICEO flow vortexes and DEP properties of the cells, allows the wireless generation of high-throughput 3D clusters or spheroids with size adjustability and biocompatibility, which shows great potential in tissue engineering, drug discovery, and tumor research.
AB - The natural environment of cells in vivo is a 3D network in which cellular responses are determined by influences from surrounding cells on all sides. This complex system is hard to be recapitulated in a conventional way. It is highly desirable to replace 2D models with 3D cell culture models. Microfluidic technology offers a variety of advantageous approaches for the long-term 3D cell culture. Herein, a new cell patterning approach for generating cell clusters of specified size and shape by exploiting induced charge electroosmosis (ICEO) flow at a bipolar electrode array is exploited. Cell or particle clusters can be produced at the edges or centers of bipolar electrodes (BPEs) with controlled organization, by designing electrodes with arbitrary shapes. This platform is further extended to flexibly organize biological units with different properties via the ICEO or dielectrophoresis (DEP) based assembly. Heterotypic cell clusters can be produced at the bipolar electrode (BPE) by adjusting the applied frequencies and the sample injection sequence. This hybrid approach by integrating ICEO flow vortexes and DEP properties of the cells, allows the wireless generation of high-throughput 3D clusters or spheroids with size adjustability and biocompatibility, which shows great potential in tissue engineering, drug discovery, and tumor research.
KW - 3D multicellular architectures
KW - bipolar electrode array
KW - induced charge electroosmosis
KW - microfluidic chip
UR - http://www.scopus.com/inward/record.url?scp=85184700616&partnerID=8YFLogxK
U2 - 10.1002/admt.202301626
DO - 10.1002/admt.202301626
M3 - 文章
AN - SCOPUS:85184700616
SN - 2365-709X
VL - 9
JO - Advanced Materials Technologies
JF - Advanced Materials Technologies
IS - 6
M1 - 2301626
ER -