Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness

Mingzhi Luo, Zhouqi Yang, Jingbao Li, Huiyun Xu, Shengsheng Li, Wei Zhang, Airong Qian, Peng Shang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We have explored the role of Ca 2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca 2+ ] i ), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca 2+ ] i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca 2+ ] i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca 2+ influx through stretch-activated channels, then results in microfilament disruption.

Original languageEnglish
Pages (from-to)2058-2068
Number of pages11
JournalAdvances in Space Research
Volume51
Issue number11
DOIs
StatePublished - 2013

Keywords

  • Calcium
  • Microfilament
  • Osteoblast
  • RPM
  • Stretch-activated channel

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