TY - JOUR
T1 - Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness
AU - Luo, Mingzhi
AU - Yang, Zhouqi
AU - Li, Jingbao
AU - Xu, Huiyun
AU - Li, Shengsheng
AU - Zhang, Wei
AU - Qian, Airong
AU - Shang, Peng
PY - 2013
Y1 - 2013
N2 - We have explored the role of Ca 2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca 2+ ] i ), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca 2+ ] i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca 2+ ] i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca 2+ influx through stretch-activated channels, then results in microfilament disruption.
AB - We have explored the role of Ca 2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca 2+ ] i ), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca 2+ ] i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca 2+ ] i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca 2+ influx through stretch-activated channels, then results in microfilament disruption.
KW - Calcium
KW - Microfilament
KW - Osteoblast
KW - RPM
KW - Stretch-activated channel
UR - http://www.scopus.com/inward/record.url?scp=84877017509&partnerID=8YFLogxK
U2 - 10.1016/j.asr.2013.01.009
DO - 10.1016/j.asr.2013.01.009
M3 - 文章
AN - SCOPUS:84877017509
SN - 0273-1177
VL - 51
SP - 2058
EP - 2068
JO - Advances in Space Research
JF - Advances in Space Research
IS - 11
ER -