Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose

Lihua Jin; Li Shang; Shaojun Guo; Youxing Fang; Dan Wen; Li Wang; Jianyuan Yin; Shaojun Dong

doi:10.1016/j.bios.2010.08.019

Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose

Lihua Jin, Li Shang, Shaojun Guo, Youxing Fang, Dan Wen, Li Wang, Jianyuan Yin, Shaojun Dong

CAS - Changchun Institute of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

254 Scopus citations

Abstract

In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H₂O₂). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0×10^-5M to 0.5×10^-3M with a detection limit of 5.0×10^-6M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes.

Original language	English
Pages (from-to)	1965-1969
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2010.08.019
State	Published - 15 Jan 2011
Externally published	Yes

Keywords

Au nanoclusters
Biomolecule
Fluorescence probe
Glucose
Hydrogen peroxide

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10.1016/j.bios.2010.08.019

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title = "Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose",

abstract = "In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H2O2). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0×10-5M to 0.5×10-3M with a detection limit of 5.0×10-6M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes.",

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AU - Jin, Lihua

AU - Shang, Li

AU - Guo, Shaojun

AU - Fang, Youxing

AU - Wen, Dan

AU - Wang, Li

AU - Yin, Jianyuan

AU - Dong, Shaojun

PY - 2011/1/15

Y1 - 2011/1/15

N2 - In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H2O2). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0×10-5M to 0.5×10-3M with a detection limit of 5.0×10-6M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes.

AB - In this work, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of glucose. The fluorescence of Au nanoclusters was found to be quenched effectively by the enzymatically generated hydrogen peroxide (H2O2). By virtue of the specific response, the present assay allowed for the selective determination of glucose in the range of 1.0×10-5M to 0.5×10-3M with a detection limit of 5.0×10-6M. The absorption spectroscopy, X-ray photoelectron spectroscopy (XPS) and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, we demonstrated the application of the present approach in real serum samples, which suggested its great potential for diagnostic purposes.

KW - Au nanoclusters

KW - Biomolecule

KW - Fluorescence probe

KW - Glucose

KW - Hydrogen peroxide

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SN - 0956-5663

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SP - 1965

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JO - Biosensors and Bioelectronics

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ER -

Biomolecule-stabilized Au nanoclusters as a fluorescence probe for sensitive detection of glucose

Abstract

Keywords

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