Application of glutaraldehyde to in-cell Western assay for normalization

Jingbao Li; Zhe Wang; Gang He; Wei Zhang; Airong Qian; Peng Shang

doi:10.1016/j.ab.2009.12.013

Application of glutaraldehyde to in-cell Western assay for normalization

Jingbao Li, Zhe Wang, Gang He, Wei Zhang, Airong Qian, Peng Shang

School of life sciences

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.

Original language	English
Pages (from-to)	254-256
Number of pages	3
Journal	Analytical Biochemistry
Volume	398
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2009.12.013
State	Published - Mar 2010

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title = "Application of glutaraldehyde to in-cell Western assay for normalization",

abstract = "Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.",

author = "Jingbao Li and Zhe Wang and Gang He and Wei Zhang and Airong Qian and Peng Shang",

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AU - Li, Jingbao

AU - Wang, Zhe

AU - He, Gang

AU - Zhang, Wei

AU - Qian, Airong

AU - Shang, Peng

PY - 2010/3

Y1 - 2010/3

N2 - Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.

AB - Normalization is essential to the in-cell Western (ICW) assay, a near-infrared immunocytoblot for protein analysis. Here we report that cells reacted with glutaraldehyde fluoresced in the near-infrared region of the spectrum, and the intensity of fluorescence was directly proportional to cell number over a range from 3125 to 100,000 cells per well. We took advantage of this property to develop a method for quantification of cells, and applied it to the ICW assay for normalization. The application of glutaraldehyde may make the ICW assay more popular due to the reduced cost and simplified procedure.

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SP - 254

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Application of glutaraldehyde to in-cell Western assay for normalization

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