Analysis of DNA and zinc finger interactions using mechanical force spectroscopy

Yanyan Wang; Shin Ichiro Oyokawa; Sung Woong Han; Wei Huang; Kazunori Ikebukuro; Chikashi Nakamura; Jun Miyake

doi:10.1007/BF02697263

Analysis of DNA and zinc finger interactions using mechanical force spectroscopy

Yanyan Wang, Shin Ichiro Oyokawa, Sung Woong Han, Wei Huang, Kazunori Ikebukuro, Chikashi Nakamura, Jun Miyake

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The unbinding force of Zif268-DNA complex has been studied by atomic force microscopy (AFM). DNA and Zif268 were covalently immobilized on the surfaces of an AFM tip and glass substrate, respectively. Confocal microscopy was used to confirm the successful immobilization of DNA. Because of the complexity of the protein-DNA interaction, parallel experiments were designed to discriminate specific interactions. For such experiments, a typical unbinding force of a single Zif268-DNA complex (approx 550 pN at 40 nN/s force loading rate) was evaluated.

Original language	English
Pages (from-to)	87-93
Number of pages	7
Journal	Nanobiotechnology
Volume	2
Issue number	3-4
DOIs	https://doi.org/10.1007/BF02697263
State	Published - Sep 2006
Externally published	Yes

Keywords

AFM
Force-distance curve
Nonspecific interaction
Protein-DNA complex
Zif268-DNA unbinding force

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10.1007/BF02697263

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title = "Analysis of DNA and zinc finger interactions using mechanical force spectroscopy",

abstract = "The unbinding force of Zif268-DNA complex has been studied by atomic force microscopy (AFM). DNA and Zif268 were covalently immobilized on the surfaces of an AFM tip and glass substrate, respectively. Confocal microscopy was used to confirm the successful immobilization of DNA. Because of the complexity of the protein-DNA interaction, parallel experiments were designed to discriminate specific interactions. For such experiments, a typical unbinding force of a single Zif268-DNA complex (approx 550 pN at 40 nN/s force loading rate) was evaluated.",

keywords = "AFM, Force-distance curve, Nonspecific interaction, Protein-DNA complex, Zif268-DNA unbinding force",

author = "Yanyan Wang and Oyokawa, {Shin Ichiro} and Han, {Sung Woong} and Wei Huang and Kazunori Ikebukuro and Chikashi Nakamura and Jun Miyake",

year = "2006",

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doi = "10.1007/BF02697263",

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T1 - Analysis of DNA and zinc finger interactions using mechanical force spectroscopy

AU - Wang, Yanyan

AU - Oyokawa, Shin Ichiro

AU - Han, Sung Woong

AU - Huang, Wei

AU - Ikebukuro, Kazunori

AU - Nakamura, Chikashi

AU - Miyake, Jun

PY - 2006/9

Y1 - 2006/9

N2 - The unbinding force of Zif268-DNA complex has been studied by atomic force microscopy (AFM). DNA and Zif268 were covalently immobilized on the surfaces of an AFM tip and glass substrate, respectively. Confocal microscopy was used to confirm the successful immobilization of DNA. Because of the complexity of the protein-DNA interaction, parallel experiments were designed to discriminate specific interactions. For such experiments, a typical unbinding force of a single Zif268-DNA complex (approx 550 pN at 40 nN/s force loading rate) was evaluated.

AB - The unbinding force of Zif268-DNA complex has been studied by atomic force microscopy (AFM). DNA and Zif268 were covalently immobilized on the surfaces of an AFM tip and glass substrate, respectively. Confocal microscopy was used to confirm the successful immobilization of DNA. Because of the complexity of the protein-DNA interaction, parallel experiments were designed to discriminate specific interactions. For such experiments, a typical unbinding force of a single Zif268-DNA complex (approx 550 pN at 40 nN/s force loading rate) was evaluated.

KW - AFM

KW - Force-distance curve

KW - Nonspecific interaction

KW - Protein-DNA complex

KW - Zif268-DNA unbinding force

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DO - 10.1007/BF02697263

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VL - 2

SP - 87

EP - 93

JO - Nanobiotechnology

JF - Nanobiotechnology

IS - 3-4

ER -

Analysis of DNA and zinc finger interactions using mechanical force spectroscopy

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Keywords

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