TY - JOUR
T1 - A Photo-Therapeutic Nanocomposite with Bio-Responsive Oxygen Self-Supplying Combats Biofilm Infections and Inflammation from Drug-Resistant Bacteria
AU - Yuan, Zhang
AU - Wu, Jianshuang
AU - Xiao, Yao
AU - Yang, Huan
AU - Meng, Siyu
AU - Dai, Liangliang
AU - Li, Peng
AU - Cai, Kaiyong
N1 - Publisher Copyright:
© 2023 Wiley-VCH GmbH.
PY - 2023/9/12
Y1 - 2023/9/12
N2 - The use of non-antibiotic strategies to combat refractory drug-resistant bacterial infections, especially biofilms and accompanying inflammation, has recently aroused widespread interest. Herein, a photo-therapeutic nanocomposite with bio-responsive oxygen (O2) self-supplying is introduced by integrating manganese dioxide (MnO2) nanozymes onto photosensitizer (indocyanine green, ICG)-loaded mesoporous polydopamine nanoparticles (MPDA), namely MI-MPDA NPs. MI-MPDA can activate O2 generation in the infection microenvironment, thereby effectively alleviating biofilm hypoxia. Under near-infrared light (NIR) irradiation, continuous O2 supplying further boosts the level of singlet oxygen (1O2), enabling robust biofilm elimination through O2-potentiated photodynamic/photothermal therapy. Interestingly, MI-MPDA down-regulates the factor expression of inflammatory signaling pathways through MnO2-mediated reactive oxygen species scavenging, which ameliorates the inflammatory condition. Meanwhile, O2 supplying prevents the M1-phenotype switch of macrophages from the overexpression of hypoxia-inducible factor-1α (HIF-1α), thereby prompting macrophage reprogramming toward pro-regenerative M2-phenotype. In the mouse models of subcutaneous implant-associated infection caused by methicillin-resistant Staphylococcus aureus (MRSA) biofilms and burn infection caused by Pseudomonas aeruginosa biofilms, NIR-irradiated MI-MPDA not only effectively eliminates the formed biofilms, but also alleviates the oxidative stress and accompanying inflammation, and drives the cascade reaction of immunomodulation-wound healing. Overall, this O2-potentiated photo-therapeutic strategy provides a reliable tool for combating biofilm infections and inflammation from drug-resistant bacteria.
AB - The use of non-antibiotic strategies to combat refractory drug-resistant bacterial infections, especially biofilms and accompanying inflammation, has recently aroused widespread interest. Herein, a photo-therapeutic nanocomposite with bio-responsive oxygen (O2) self-supplying is introduced by integrating manganese dioxide (MnO2) nanozymes onto photosensitizer (indocyanine green, ICG)-loaded mesoporous polydopamine nanoparticles (MPDA), namely MI-MPDA NPs. MI-MPDA can activate O2 generation in the infection microenvironment, thereby effectively alleviating biofilm hypoxia. Under near-infrared light (NIR) irradiation, continuous O2 supplying further boosts the level of singlet oxygen (1O2), enabling robust biofilm elimination through O2-potentiated photodynamic/photothermal therapy. Interestingly, MI-MPDA down-regulates the factor expression of inflammatory signaling pathways through MnO2-mediated reactive oxygen species scavenging, which ameliorates the inflammatory condition. Meanwhile, O2 supplying prevents the M1-phenotype switch of macrophages from the overexpression of hypoxia-inducible factor-1α (HIF-1α), thereby prompting macrophage reprogramming toward pro-regenerative M2-phenotype. In the mouse models of subcutaneous implant-associated infection caused by methicillin-resistant Staphylococcus aureus (MRSA) biofilms and burn infection caused by Pseudomonas aeruginosa biofilms, NIR-irradiated MI-MPDA not only effectively eliminates the formed biofilms, but also alleviates the oxidative stress and accompanying inflammation, and drives the cascade reaction of immunomodulation-wound healing. Overall, this O2-potentiated photo-therapeutic strategy provides a reliable tool for combating biofilm infections and inflammation from drug-resistant bacteria.
KW - anti-inflammation
KW - biofilms
KW - drug-resistant bacteria
KW - immunomodulation
KW - oxygen-potentiated photo-therapy
UR - http://www.scopus.com/inward/record.url?scp=85159913091&partnerID=8YFLogxK
U2 - 10.1002/adfm.202302908
DO - 10.1002/adfm.202302908
M3 - 文章
AN - SCOPUS:85159913091
SN - 1616-301X
VL - 33
JO - Advanced Functional Materials
JF - Advanced Functional Materials
IS - 37
M1 - 2302908
ER -