A new approach to produce resveratrol by enzymatic bioconversion

Jinxin Che, Junling Shi, Zhenhong Gao, Yan Zhang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 μg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.

Original languageEnglish
Pages (from-to)1348-1357
Number of pages10
JournalJournal of Microbiology and Biotechnology
Volume26
Issue number8
DOIs
StatePublished - Aug 2016

Keywords

  • Alternaria sp
  • Enzymatic synthesis
  • Immobilized enzyme
  • Resveratrol

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