TY - JOUR
T1 - A new approach to produce resveratrol by enzymatic bioconversion
AU - Che, Jinxin
AU - Shi, Junling
AU - Gao, Zhenhong
AU - Zhang, Yan
N1 - Publisher Copyright:
© 2016 by The Korean Society for Microbiology and Biotechnology.
PY - 2016/8
Y1 - 2016/8
N2 - An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 μg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
AB - An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 μg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
KW - Alternaria sp
KW - Enzymatic synthesis
KW - Immobilized enzyme
KW - Resveratrol
UR - http://www.scopus.com/inward/record.url?scp=84984844793&partnerID=8YFLogxK
U2 - 10.4014/jmb.1512.12084
DO - 10.4014/jmb.1512.12084
M3 - 文章
C2 - 27116990
AN - SCOPUS:84984844793
SN - 1017-7825
VL - 26
SP - 1348
EP - 1357
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 8
ER -