A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia

Wen Lv; Zhang Zhang; Kenneth Yin Zhang; Huiran Yang; Shujuan Liu; Aqiang Xu; Song Guo; Qiang Zhao; Wei Huang

doi:10.1002/anie.201604130

A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia

Wen Lv, Zhang Zhang, Kenneth Yin Zhang, Huiran Yang, Shujuan Liu, Aqiang Xu, Song Guo, Qiang Zhao, Wei Huang

Research output: Contribution to journal › Article › peer-review

465 Scopus citations

Abstract

Organelle-targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria and lysosomes of living cells, respectively. Both complexes exhibited long-lived phosphorescence, which is sensitive to oxygen quenching. The photocytotoxicity of the complexes was evaluated under normoxic and hypoxic conditions. The results showed that HeLa cells treated with the mitochondria-targeted complex maintained a slower respiration rate, leading to a higher intracellular oxygen level under hypoxia. As a result, this complex exhibited an improved PDT effect compared to the lysosome-targeted complex, especially under hypoxia conditions, suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.

Original language	English
Pages (from-to)	9947-9951
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	55
Issue number	34
DOIs	https://doi.org/10.1002/anie.201604130
State	Published - 16 Aug 2016
Externally published	Yes

Keywords

cell death
hypoxia
iridium
photodynamic therapy
singlet oxygen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201604130

Cite this

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title = "A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia",

abstract = "Organelle-targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria and lysosomes of living cells, respectively. Both complexes exhibited long-lived phosphorescence, which is sensitive to oxygen quenching. The photocytotoxicity of the complexes was evaluated under normoxic and hypoxic conditions. The results showed that HeLa cells treated with the mitochondria-targeted complex maintained a slower respiration rate, leading to a higher intracellular oxygen level under hypoxia. As a result, this complex exhibited an improved PDT effect compared to the lysosome-targeted complex, especially under hypoxia conditions, suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.",

keywords = "cell death, hypoxia, iridium, photodynamic therapy, singlet oxygen",

author = "Wen Lv and Zhang Zhang and Zhang, {Kenneth Yin} and Huiran Yang and Shujuan Liu and Aqiang Xu and Song Guo and Qiang Zhao and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2016",

month = aug,

day = "16",

doi = "10.1002/anie.201604130",

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T1 - A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia

AU - Lv, Wen

AU - Zhang, Zhang

AU - Zhang, Kenneth Yin

AU - Yang, Huiran

AU - Liu, Shujuan

AU - Xu, Aqiang

AU - Guo, Song

AU - Zhao, Qiang

AU - Huang, Wei

PY - 2016/8/16

Y1 - 2016/8/16

N2 - Organelle-targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria and lysosomes of living cells, respectively. Both complexes exhibited long-lived phosphorescence, which is sensitive to oxygen quenching. The photocytotoxicity of the complexes was evaluated under normoxic and hypoxic conditions. The results showed that HeLa cells treated with the mitochondria-targeted complex maintained a slower respiration rate, leading to a higher intracellular oxygen level under hypoxia. As a result, this complex exhibited an improved PDT effect compared to the lysosome-targeted complex, especially under hypoxia conditions, suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.

AB - Organelle-targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria and lysosomes of living cells, respectively. Both complexes exhibited long-lived phosphorescence, which is sensitive to oxygen quenching. The photocytotoxicity of the complexes was evaluated under normoxic and hypoxic conditions. The results showed that HeLa cells treated with the mitochondria-targeted complex maintained a slower respiration rate, leading to a higher intracellular oxygen level under hypoxia. As a result, this complex exhibited an improved PDT effect compared to the lysosome-targeted complex, especially under hypoxia conditions, suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.

KW - cell death

KW - hypoxia

KW - iridium

KW - photodynamic therapy

KW - singlet oxygen

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U2 - 10.1002/anie.201604130

DO - 10.1002/anie.201604130

M3 - 文章

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 34

ER -

A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia

Abstract

Keywords

UN SDGs

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