强化细胞外排提高工程菌类黄酮产量的策略

Flavonoid has a broad spectrum of antibacterial activity and high antioxidant activity, showing extensive usage and large market in food and biomedical fields, but there are many disadvantages in the currently used methods to extract flavonoid from plants. Thus microbial fermentation has become the most potential technique to produce flavonoid around the world. The main current strategy to solve this issue is to construct high-yield strains through metabolic pathway modification and gene optimization, but the antibacterial effect of flavonoid on the producing strain was little considered . Considering the antibacterial activity of flavonoid may inhibit the bacterial growth and thus limit the flavonoid production of microbial fermentation, we proposed that introducing or strengthening the flavonoid efflux may be helpful to enhancing the flavonoid production of microbial fermentation, based on the flavonoid efflux mechanism and key genes in animals, plants and microorganisms, as well as combined with cases of the relevant genes that have been successfully expressed in genetically modified bacteria, aiming to provide useful information for the construction of high-flavonoid-producing microorganisms.