基于GMA-IDA-Cu2+螯合作用识别BSA的温敏印迹聚合物

Xiang Zhi Dong; Yong Ma; Chun Ping Hou; Bao Liang Zhang; He Peng Zhang; Qiu Yu Zhang

doi:10.16606/j.cnki.issn0253-4320.2020.01.029

基于GMA-IDA-Cu²⁺螯合作用识别BSA的温敏印迹聚合物

Translated title of the contribution: Thermo-sensitive MIP for recognition of bovine serum albumin based on chelating performance of GMA-IDA-Cu²⁺

Xiang Zhi Dong, Yong Ma, Chun Ping Hou, Bao Liang Zhang, He Peng Zhang, Qiu Yu Zhang

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A thermo-sensitive molecularly imprinted polymer (MIP) is synthesized through graft copolymerization of "whisker" polymer chains on the surface of modified SiO₂ and then copolymerization with thermo-sensitive monomers (NIPAM). The template protein bovine serum albumin (BSA) is selectively adsorbed by GMA-IDA-Cu²⁺ by means of metal chelation. TEM, IR and TGA measurements prove that the imprinted layer has been grafted onto SiO₂ outer layer, and the thickness of the imprinted layer is around 9 nm. The optimum experiments for pH and thermo-sensitive conditions verify that MIP has an excellent temperature sensitivity. An adsorption capacity as high as 115.42 mg•g^-1 and an imprinting factor of 2.58) are obtained at pH 4.6. Specific adsorption tests for different proteins show that the imprinted cavities and imprinted sites inside the MIP match well with the template protein BSA. In addition, the MIP also exhibits higher adsorption rate and better repeatability.

Translated title of the contribution	Thermo-sensitive MIP for recognition of bovine serum albumin based on chelating performance of GMA-IDA-Cu²⁺
Original language	Chinese (Traditional)
Pages (from-to)	134-139
Number of pages	6
Journal	Xiandai Huagong/Modern Chemical Industry
Volume	40
Issue number	1
DOIs	https://doi.org/10.16606/j.cnki.issn0253-4320.2020.01.029
State	Published - 20 Jan 2020

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@article{27ee2236924d4c68a76deb7cf4945540,

title = "基于GMA-IDA-Cu2+螯合作用识别BSA的温敏印迹聚合物",

abstract = "A thermo-sensitive molecularly imprinted polymer (MIP) is synthesized through graft copolymerization of {"}whisker{"} polymer chains on the surface of modified SiO2 and then copolymerization with thermo-sensitive monomers (NIPAM). The template protein bovine serum albumin (BSA) is selectively adsorbed by GMA-IDA-Cu2+ by means of metal chelation. TEM, IR and TGA measurements prove that the imprinted layer has been grafted onto SiO2 outer layer, and the thickness of the imprinted layer is around 9 nm. The optimum experiments for pH and thermo-sensitive conditions verify that MIP has an excellent temperature sensitivity. An adsorption capacity as high as 115.42 mg•g-1 and an imprinting factor of 2.58) are obtained at pH 4.6. Specific adsorption tests for different proteins show that the imprinted cavities and imprinted sites inside the MIP match well with the template protein BSA. In addition, the MIP also exhibits higher adsorption rate and better repeatability.",

keywords = "Adsorption properties, Bovine serum albumin, Chelation, Molecular imprinting polymer, Thermo-sensitivity",

author = "Dong, {Xiang Zhi} and Yong Ma and Hou, {Chun Ping} and Zhang, {Bao Liang} and Zhang, {He Peng} and Zhang, {Qiu Yu}",

year = "2020",

month = jan,

day = "20",

doi = "10.16606/j.cnki.issn0253-4320.2020.01.029",

language = "繁体中文",

volume = "40",

pages = "134--139",

journal = "Xiandai Huagong/Modern Chemical Industry",

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publisher = "China National Chemical Information Center",

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T1 - 基于GMA-IDA-Cu2+螯合作用识别BSA的温敏印迹聚合物

AU - Dong, Xiang Zhi

AU - Ma, Yong

AU - Hou, Chun Ping

AU - Zhang, Bao Liang

AU - Zhang, He Peng

AU - Zhang, Qiu Yu

PY - 2020/1/20

Y1 - 2020/1/20

N2 - A thermo-sensitive molecularly imprinted polymer (MIP) is synthesized through graft copolymerization of "whisker" polymer chains on the surface of modified SiO2 and then copolymerization with thermo-sensitive monomers (NIPAM). The template protein bovine serum albumin (BSA) is selectively adsorbed by GMA-IDA-Cu2+ by means of metal chelation. TEM, IR and TGA measurements prove that the imprinted layer has been grafted onto SiO2 outer layer, and the thickness of the imprinted layer is around 9 nm. The optimum experiments for pH and thermo-sensitive conditions verify that MIP has an excellent temperature sensitivity. An adsorption capacity as high as 115.42 mg•g-1 and an imprinting factor of 2.58) are obtained at pH 4.6. Specific adsorption tests for different proteins show that the imprinted cavities and imprinted sites inside the MIP match well with the template protein BSA. In addition, the MIP also exhibits higher adsorption rate and better repeatability.

AB - A thermo-sensitive molecularly imprinted polymer (MIP) is synthesized through graft copolymerization of "whisker" polymer chains on the surface of modified SiO2 and then copolymerization with thermo-sensitive monomers (NIPAM). The template protein bovine serum albumin (BSA) is selectively adsorbed by GMA-IDA-Cu2+ by means of metal chelation. TEM, IR and TGA measurements prove that the imprinted layer has been grafted onto SiO2 outer layer, and the thickness of the imprinted layer is around 9 nm. The optimum experiments for pH and thermo-sensitive conditions verify that MIP has an excellent temperature sensitivity. An adsorption capacity as high as 115.42 mg•g-1 and an imprinting factor of 2.58) are obtained at pH 4.6. Specific adsorption tests for different proteins show that the imprinted cavities and imprinted sites inside the MIP match well with the template protein BSA. In addition, the MIP also exhibits higher adsorption rate and better repeatability.

KW - Adsorption properties

KW - Bovine serum albumin

KW - Chelation

KW - Molecular imprinting polymer

KW - Thermo-sensitivity

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U2 - 10.16606/j.cnki.issn0253-4320.2020.01.029

DO - 10.16606/j.cnki.issn0253-4320.2020.01.029

M3 - 文章

AN - SCOPUS:85078857802

SN - 0253-4320

VL - 40

SP - 134

EP - 139

JO - Xiandai Huagong/Modern Chemical Industry

JF - Xiandai Huagong/Modern Chemical Industry

IS - 1

ER -

基于GMA-IDA-Cu²⁺螯合作用识别BSA的温敏印迹聚合物

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