TY - JOUR
T1 - Transplantation of olfactory ensheathing cells embedded in the GelMA-F127DA hydrogel promotes the repair of spinal cord injury
AU - Yang, Yubing
AU - Li, Jiaxi
AU - Zhao, Jinghao
AU - Guo, Dong
AU - Li, Jing
AU - Li, Jialiang
AU - Zhang, Chengyi
AU - Zhou, Chengdong
AU - Hao, Wanzhu
AU - Li, Juan
AU - Ye, Shengshou
AU - Chang, Su’e
AU - Yi, Gaohang
AU - Zheng, Hua
AU - Zhang, Qiuyu
AU - He, Xijing
N1 - Publisher Copyright:
This journal is © The Royal Society of Chemistry, 2025
PY - 2025/12/28
Y1 - 2025/12/28
N2 - Spinal cord injury (SCI) is a highly disabling condition. Olfactory ensheathing cells (OECs) are important transplantable cells for treating spinal cord injury (SCI). However, directly transplanting cells into the injured area may result in the loss of transplanted cells. Additionally, secondary inflammatory responses reduce the survival of transplanted cells. In this study, a hydrogel with favorable properties, including plasticity, strong adhesion, mechanical stability, shear-thinning behavior, and pore size suitable for OEC growth and axon extension, was fabricated from methacryloylated gelatin-pluronic F127 diacrylate (GelMA-F127DA or GEF). In vitro results showed that the GEF hydrogel possessed excellent biocompatibility. Importantly, animal experiments confirmed that transplantation of OECs embedded in the GEF hydrogel effectively repaired SCI, promoted neuronal regeneration and axon remyelination, and improved locomotor function. Accordingly, this treatment strategy offers a new perspective for SCI repair.
AB - Spinal cord injury (SCI) is a highly disabling condition. Olfactory ensheathing cells (OECs) are important transplantable cells for treating spinal cord injury (SCI). However, directly transplanting cells into the injured area may result in the loss of transplanted cells. Additionally, secondary inflammatory responses reduce the survival of transplanted cells. In this study, a hydrogel with favorable properties, including plasticity, strong adhesion, mechanical stability, shear-thinning behavior, and pore size suitable for OEC growth and axon extension, was fabricated from methacryloylated gelatin-pluronic F127 diacrylate (GelMA-F127DA or GEF). In vitro results showed that the GEF hydrogel possessed excellent biocompatibility. Importantly, animal experiments confirmed that transplantation of OECs embedded in the GEF hydrogel effectively repaired SCI, promoted neuronal regeneration and axon remyelination, and improved locomotor function. Accordingly, this treatment strategy offers a new perspective for SCI repair.
UR - https://www.scopus.com/pages/publications/105022739399
U2 - 10.1039/d5tb01678a
DO - 10.1039/d5tb01678a
M3 - 文章
AN - SCOPUS:105022739399
SN - 2050-750X
VL - 13
SP - 15620
EP - 15632
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 48
ER -