The Morphology Deformation Simulation of BV-2 Cells with Elevated Intracranial Pressure Under Space Microgravity Conditions

The elevated intracranial pressure under microgravity conditions will lead to the variation of physical structure and physiological behavior of brain cells. In this work, a three-dimensional numerical simulation model of BV-2 cells adhered to the substrate with different morphologies is constructed, the cell deformation under typical space microgravity conditions is simulated and the cell function response under corresponding conditions are experimentally analyzed. The mechanical properties of cells are obtained by atomic force microscopy testing. The effects of cell height and intracranial pressure which corresponds to certain microgravity condition on the cell deformation are clarified. The results demonstrate that the model can accurately calculate both the mechanical and displacement responses of the cell following a change in external pressure. This simulation enables the prediction of the concentration area of the stress, the amount of deformation and the potential risk of rupture of adherent cells with different morphologies under varying pressures.