TY - JOUR
T1 - Small-Molecule Quenchers for Förster Resonance Energy Transfer
T2 - Structure, Mechanism, and Applications
AU - Fang, Bin
AU - Shen, Yu
AU - Peng, Bo
AU - Bai, Hua
AU - Wang, Limin
AU - Zhang, Jiaxin
AU - Hu, Wenbo
AU - Fu, Li
AU - Zhang, Wei
AU - Li, Lin
AU - Huang, Wei
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/10/10
Y1 - 2022/10/10
N2 - For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely used mechanisms to realize the detection of biochemical activities. Dark quenchers relax from the excited state to the ground state non-radiatively, and are promising alternatives to fluorescent FRET acceptors. Small-molecule (dark) quenchers have been widely used as acceptors in FRET-based probes to monitor various physiological processes with minimum background signal. Herein, we summarize the relevant advances of small-molecule quenchers that are used in FRET-based probes. This Review is intended to provide suggestions regarding the rationale design and selection of appropriate fluorophore–quencher FRET pairs, which are fully compatible with challenging analytical applications in various biological systems. Finally, an outlook of the future biomedical applications and developments of this field is presented.
AB - For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely used mechanisms to realize the detection of biochemical activities. Dark quenchers relax from the excited state to the ground state non-radiatively, and are promising alternatives to fluorescent FRET acceptors. Small-molecule (dark) quenchers have been widely used as acceptors in FRET-based probes to monitor various physiological processes with minimum background signal. Herein, we summarize the relevant advances of small-molecule quenchers that are used in FRET-based probes. This Review is intended to provide suggestions regarding the rationale design and selection of appropriate fluorophore–quencher FRET pairs, which are fully compatible with challenging analytical applications in various biological systems. Finally, an outlook of the future biomedical applications and developments of this field is presented.
KW - Activity-Based Probes
KW - Bio-Imaging
KW - Bio-Molecular Assays
KW - Förster Resonance Energy Transfer (FRET)
KW - Quenchers
UR - https://www.scopus.com/pages/publications/85137606674
U2 - 10.1002/anie.202207188
DO - 10.1002/anie.202207188
M3 - 文献综述
C2 - 35852404
AN - SCOPUS:85137606674
SN - 1433-7851
VL - 61
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 41
M1 - e202207188
ER -