TY - JOUR
T1 - Single base-resolution methylome of the dizygotic sheep
AU - Wang, Yangzi
AU - Wu, Jianghong
AU - Ma, Xiao
AU - Liu, Bin
AU - Su, Rui
AU - Jiang, Yu
AU - Wang, Wen
AU - Dong, Yang
N1 - Publisher Copyright:
© 2015 Wang et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2015/11/4
Y1 - 2015/11/4
N2 - Sheep is an important livestock in the world for meat, dairy and wool production. The third version of sheep reference genome has been recently assembled, but sheep DNA methylome has not been profiled yet. In this study, we report the comprehensive sheep methylome with 94.38% cytosine coverage at single base resolution by sequencing DNA samples from Longissimus dorsi of dizygotic Sunit sheep, which were bred in different habitats. We also compared methylomes between the twin sheep. DNA methylation status at genomescale differentially methylated regions (DMRs), functional genomic regions and 248 DMRcontaining genes were identified between the twin sheep. Gene ontology (GO) and KEGG annotations of these genes were performed to discover computationally predicted function. Lipid metabolism, sexual maturity and tumor-associated categories were observed to significantly enrich DMR-containing genes. These findings could be used to illustrate the relationship between phenotypic variations and gene methylation patterns.
AB - Sheep is an important livestock in the world for meat, dairy and wool production. The third version of sheep reference genome has been recently assembled, but sheep DNA methylome has not been profiled yet. In this study, we report the comprehensive sheep methylome with 94.38% cytosine coverage at single base resolution by sequencing DNA samples from Longissimus dorsi of dizygotic Sunit sheep, which were bred in different habitats. We also compared methylomes between the twin sheep. DNA methylation status at genomescale differentially methylated regions (DMRs), functional genomic regions and 248 DMRcontaining genes were identified between the twin sheep. Gene ontology (GO) and KEGG annotations of these genes were performed to discover computationally predicted function. Lipid metabolism, sexual maturity and tumor-associated categories were observed to significantly enrich DMR-containing genes. These findings could be used to illustrate the relationship between phenotypic variations and gene methylation patterns.
UR - https://www.scopus.com/pages/publications/84951188509
U2 - 10.1371/journal.pone.0142034
DO - 10.1371/journal.pone.0142034
M3 - 文章
C2 - 26536671
AN - SCOPUS:84951188509
SN - 1932-6203
VL - 10
JO - PLoS ONE
JF - PLoS ONE
IS - 11
M1 - e0142034
ER -