Inhibitory effects of constant magnetic field on secretion of intercellular adhesion molecule-1 by endothelial cells and their adhesion with monocytes

Objective: To investigate the effects of constant magnetic field on secretion and expression of intercellular adhesion molecule-1 (ICAM-1) by human umbilical vein endothelial cells (HUVEC) and their adhesion with monocytes (THP-1) induced by oxidized low-density lipoprotein (OX-LDL). Methods: The third passage of cultured HUVECs were used. The cells were divided into six groups: control group, OX-LDL group, and OX-LDL with one, five, ten or 20 gausses of constant magnetic field groups. Samples were collected 24 hours later. Secretion of ICAM-1 was detected by ELISA, and expression of ICAM-1 was detected by immunocytochemistry. Adhesion rates between HUVEC and THP-1 was measured by counting method. Results: Secretion of ICAM-1 significantly increased 24 hours after intervention by OX-LDL of 100 mg·L^-1 (P<0.05 vs. control). However, in one, five, ten and 20 gausses groups, secretion and expression of ICAM-1 significantly decreased compared with those of OX-LDL group (P < 0.05). The adhesion rates between HUVEC and THP-1 significantly increased 24 hours after incubation of HUVEC with OX-LDL (P<0.05 vs. control). On the other hand, in one, five, ten and 20 gausses groups, adhesion rates between HUVEC and THP-1 significantly decreased compared with those of the OX-LDL group (P < 0.05). Conclusion: One gauss to 20 gausses of constant magnetic fields can intensity-dependently antagonize effects of OX-LDL on HUVEC by decreasing secretion and expression of ICAM-1 by HUVEC and decreasing adhesion rates between HUVEC and THP-1.