Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

Liulin Wang; Buxiang Chen; Pingping Peng; Wenbo Hu; Zhipeng Liu; Xiaohua Pei; Weihong Zhao; Chengwu Zhang; Lin Li; Wei Huang

doi:10.1016/j.cclet.2017.07.016

Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

Liulin Wang
, Buxiang Chen
, Pingping Peng
, Wenbo Hu
, Zhipeng Liu
, Xiaohua Pei
, Weihong Zhao
, Chengwu Zhang
, Lin Li
, Wei Huang

Nanjing Tech University
Nanjing Medical University
Nanjing University of Posts and Telecommunications

科研成果: 期刊稿件 › 文章 › 同行评审

27 引用（Scopus）

摘要

Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, M_Cu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10⁻⁷ mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated M_Cu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

源语言	英语
页（从-至）	1965-1968
页数	4
期刊	Chinese Chemical Letters
卷	28
期	10
DOI	https://doi.org/10.1016/j.cclet.2017.07.016
出版状态	已出版 - 10月 2017
已对外发布	是

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title = "Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells",

abstract = "Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.",

keywords = "Copper(II), Fluorescence imaging, Fluorogenic probe, Mitochondria, Photocontrollable",

author = "Liulin Wang and Buxiang Chen and Pingping Peng and Wenbo Hu and Zhipeng Liu and Xiaohua Pei and Weihong Zhao and Chengwu Zhang and Lin Li and Wei Huang",

note = "Publisher Copyright: {\textcopyright} 2017 The Author",

year = "2017",

month = oct,

doi = "10.1016/j.cclet.2017.07.016",

language = "英语",

volume = "28",

pages = "1965--1968",

journal = "Chinese Chemical Letters",

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TY - JOUR

T1 - Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

AU - Wang, Liulin

AU - Chen, Buxiang

AU - Peng, Pingping

AU - Hu, Wenbo

AU - Liu, Zhipeng

AU - Pei, Xiaohua

AU - Zhao, Weihong

AU - Zhang, Chengwu

AU - Li, Lin

AU - Huang, Wei

PY - 2017/10

Y1 - 2017/10

N2 - Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

AB - Monitoring mitochondrial derived copper(II) in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper(II). Herein, we have reported the design, synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group (nitrobenzyl group) and mitochondria targeting unit (triphenylphosphonium salt). This novel probe showed an intense fluorescence enhancement in response to copper(II) without interference from other metal cations in the biological condition (pH 6–9). The detection limit is 1.7 × 10−7 mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper(II) in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper(II) during mitochondria specific detection.

KW - Copper(II)

KW - Fluorescence imaging

KW - Fluorogenic probe

KW - Mitochondria

KW - Photocontrollable

UR - https://www.scopus.com/pages/publications/85026763732

U2 - 10.1016/j.cclet.2017.07.016

DO - 10.1016/j.cclet.2017.07.016

M3 - 文章

AN - SCOPUS:85026763732

SN - 1001-8417

VL - 28

SP - 1965

EP - 1968

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 10

ER -

Fluorescence imaging mitochondrial copper(II) via photocontrollable fluorogenic probe in live cells

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