A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou; Bo Wang; Shan Yang Hu; Jing Zhang Wang; Peng Fei Zhu; Yue Liu; Meng Ying Wang; Da Chuan Yin

doi:10.1039/c5ce00551e

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

Hai Hou
, Bo Wang
, Shan Yang Hu
, Jing Zhang Wang
, Peng Fei Zhu
, Yue Liu
, Meng Ying Wang
, Da Chuan Yin

生命学院

Northwestern Polytechnical University Xian
Hebei University of Engineering

科研成果: 期刊稿件 › 文章 › 同行评审

9 引用（Scopus）

摘要

We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

源语言	英语
页（从-至）	5365-5371
页数	7
期刊	CrystEngComm
卷	17
期	29
DOI	https://doi.org/10.1039/c5ce00551e
出版状态	已出版 - 7 8月 2015

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abstract = "We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.",

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AU - Hou, Hai

AU - Wang, Bo

AU - Hu, Shan Yang

AU - Wang, Jing Zhang

AU - Zhu, Peng Fei

AU - Liu, Yue

AU - Wang, Meng Ying

AU - Yin, Da Chuan

PY - 2015/8/7

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N2 - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

AB - We presented a systematic quality comparison of protein crystals grown using the cross-diffusion microbatch (CDM) and standard sitting-drop vapor diffusion methods. Eleven proteins were screened and it was found that crystals grown using CDM exhibited a better morphology. X-ray diffraction showed that the CDM method is practical and useful for obtaining high-quality protein crystals.

UR - https://www.scopus.com/pages/publications/84937053863

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ER -

A comparative study on the quality of protein crystals obtained using the cross-diffusion microbatch and sitting-drop vapor diffusion methods

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