TY - JOUR
T1 - Versatile digital polymerase chain reaction chip design, fabrication, and image processing
AU - Li, Huanan
AU - Zhang, Haoqing
AU - Xu, Ying
AU - Tureckova, Alzbeta
AU - Zahradník, Pavel
AU - Chang, Honglong
AU - Neuzil, Pavel
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/15
Y1 - 2019/3/15
N2 - We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.
AB - We present a family of digital polymerase chain reaction (dPCR) chips made of silicon that are (9 × 9) mm2 in size, have a unique layout design, and have between 26,448 and 1,656,000 reaction wells. The chip layout was split into six fields sized 4200 × 2785 μm2 that contain reaction wells with a gap of a few μm separating the fields from each other. We also developed a universal fluorescence imaging method capable of processing images from those chips. It comprised automatic image acquisition, image stitching, alignment mark registration, rotation correction, determination of reaction wells’ positions, and result processing. The chip design, with fully automated image processing, provided a fast and reliable system for the absolute quantitative analysis of dPCR. Combined with ease-of-use, this family of dPCR chips offers a pathway to enable fast and affordable digital microfluidic diagnostic applications.
KW - Array
KW - Digital PCR
KW - DNA counting
KW - Fluorescence image analysis
KW - Microfluidic chip
KW - Silicon chip
UR - http://www.scopus.com/inward/record.url?scp=85058703025&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2018.12.072
DO - 10.1016/j.snb.2018.12.072
M3 - 文章
AN - SCOPUS:85058703025
SN - 0925-4005
VL - 283
SP - 677
EP - 684
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -