Unique cytological behavior of MC3T3-E1 osteoblasts on H2O2-modified C/C composites in vitro

Sheng Cao; Hejun Li; Jinhua Lu; Leilei Zhang

doi:10.1007/s40843-016-9015-9

Unique cytological behavior of MC3T3-E1 osteoblasts on H₂O₂-modified C/C composites in vitro

Sheng Cao
, Hejun Li
, Jinhua Lu
, Leilei Zhang

School of Materials Sience and Engineering

Northwestern Polytechnical University Xian

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

MC3T3-E1 osteoblasts were cultured on H₂O₂-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.

Original language	English
Pages (from-to)	361-367
Number of pages	7
Journal	Science China Materials
Volume	60
Issue number	4
DOIs	https://doi.org/10.1007/s40843-016-9015-9
State	Published - 1 Apr 2017

Keywords

HO modification
MC3T3-E1 osteoblasts
carbon/carbon composites
surface wettability

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10.1007/s40843-016-9015-9

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title = "Unique cytological behavior of MC3T3-E1 osteoblasts on H2O2-modified C/C composites in vitro",

abstract = "MC3T3-E1 osteoblasts were cultured on H2O2-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.",

keywords = "HO modification, MC3T3-E1 osteoblasts, carbon/carbon composites, surface wettability",

author = "Sheng Cao and Hejun Li and Jinhua Lu and Leilei Zhang",

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doi = "10.1007/s40843-016-9015-9",

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TY - JOUR

T1 - Unique cytological behavior of MC3T3-E1 osteoblasts on H2O2-modified C/C composites in vitro

AU - Cao, Sheng

AU - Li, Hejun

AU - Lu, Jinhua

AU - Zhang, Leilei

PY - 2017/4/1

Y1 - 2017/4/1

N2 - MC3T3-E1 osteoblasts were cultured on H2O2-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.

AB - MC3T3-E1 osteoblasts were cultured on H2O2-modified and unmodified carbon/carbon (H-C/C and C/C) composites for one week in order to evaluate differences in cell adhesion, spreading, and proliferation. The results indicated a certain degree of enhancement in the cell adhesion capability of osteoblasts cultured on H-C/C samples. Cellular morphologies after cell adhesion were observed via scanning electron microscopy (SEM), which showed that the cells adhered more closely and spread more widely on the H-C/C sample surface. However, no cell appeared in several multiple and continuous types of minor pores on both the C/C and H-C/C surfaces. In addition, two unique situations were observed on the H-C/C samples: an outline change of the osteoblasts was observed when the cells spread across some minor pores, and the cells entered and adhered well in some larger pores.

KW - HO modification

KW - MC3T3-E1 osteoblasts

KW - carbon/carbon composites

KW - surface wettability

UR - https://www.scopus.com/pages/publications/85035110014

U2 - 10.1007/s40843-016-9015-9

DO - 10.1007/s40843-016-9015-9

M3 - 文章

AN - SCOPUS:85035110014

SN - 2095-8226

VL - 60

SP - 361

EP - 367

JO - Science China Materials

JF - Science China Materials

IS - 4

ER -

Unique cytological behavior of MC3T3-E1 osteoblasts on H₂O₂-modified C/C composites in vitro

Abstract

Keywords

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