Synthesis of novel gold mesoflowers as SERS tags for immunoassay with improved sensitivity

A new class of flowerlike gold mesostructure in high yield is successfully synthesized through a facile one-step route using ascorbic acid as a reducing agent of gold salt with cetyltrimethylammonium chloride (CTAC) as surfactant. The as-prepared Au particles have spherical profiles with an averaged diameter of 770 ± 50 nm, but showing a highly rough surface consisting of many irregular and randomly arranged protrusions. The Au mesoflowers exhibit strong surface-enhanced effects and near-infrared absorption which were utilized in the design of efficient surface-enhanced Raman scattering (SERS) tags as immunosensors for immunoassay with improved sensitivity. The experimental results indicate that a good linear relationship is found between the peak intensity at 1071 cm^-1 and the logarithm of H-IgG concentration in the range between 1 ng/mL and 1 fg/mL, and the limit of detection (LOD) is 1 fg/mL.