Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

Zhen Li; Liang Sun; Yufei Zhang; Andrew P. Dove; Rachel K. O'Reilly; Guosong Chen

doi:10.1021/acsmacrolett.6b00419

Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

Zhen Li, Liang Sun, Yufei Zhang, Andrew P. Dove, Rachel K. O'Reilly, Guosong Chen

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (L_n × W_n = 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (L_n × W_n = 99 × 50 nm) and spherical GNPs (D_n = 46 nm).

Original language	English
Pages (from-to)	1059-1064
Number of pages	6
Journal	ACS Macro Letters
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/acsmacrolett.6b00419
State	Published - 20 Sep 2016
Externally published	Yes

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title = "Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response",

abstract = "The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (Ln × Wn = 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (Ln × Wn = 99 × 50 nm) and spherical GNPs (Dn = 46 nm).",

author = "Zhen Li and Liang Sun and Yufei Zhang and Dove, {Andrew P.} and O'Reilly, {Rachel K.} and Guosong Chen",

note = "Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

year = "2016",

month = sep,

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doi = "10.1021/acsmacrolett.6b00419",

language = "英语",

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T1 - Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

AU - Li, Zhen

AU - Sun, Liang

AU - Zhang, Yufei

AU - Dove, Andrew P.

AU - O'Reilly, Rachel K.

AU - Chen, Guosong

PY - 2016/9/20

Y1 - 2016/9/20

N2 - The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (Ln × Wn = 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (Ln × Wn = 99 × 50 nm) and spherical GNPs (Dn = 46 nm).

AB - The shells of various poly(dl-lactide)-b-poly(acrylic acid) (PDLLA-b-PAA) spherical micelles and poly(l-lactide)-b-poly(acrylic acid) (PLLA-b-PAA) cylindrical micelles were functionalized with mannose to yield glyco-nanoparticles (GNPs) with different shapes and dimensions. All of these GNPs were shown to have good biocompatibility (up to 1 mg/mL). Cellular uptake experiments using RAW 264.7 have shown that the spherical GNPs were internalized to a much greater extent than the cylindrical GNPs and such a phenomenon was attributed to their different endocytosis pathways. It was demonstrated that spherical GNPs were internalized based on clathrin- and caveolin-mediated endocytosis while cylindrical GNPs mainly depended on clathrin-mediated endocytosis. We also found that longer cylindrical GNPs (Ln × Wn = 215 × 47 nm) can induce an inflammatory response (specifically interleukin 6) more efficiently than shorter cylindrical GNPs (Ln × Wn = 99 × 50 nm) and spherical GNPs (Dn = 46 nm).

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U2 - 10.1021/acsmacrolett.6b00419

DO - 10.1021/acsmacrolett.6b00419

M3 - 文章

AN - SCOPUS:84988603453

SN - 2161-1653

VL - 5

SP - 1059

EP - 1064

JO - ACS Macro Letters

JF - ACS Macro Letters

IS - 9

ER -

Shape Effect of Glyco-Nanoparticles on Macrophage Cellular Uptake and Immune Response

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