Preparation and specific adsorption of dual antibacterial BSA surface imprinted GO-PEI/MXene wrinkled microspheres

Molecular imprinting technology has gained increasing attention and application in protein adsorption and separation. Bacterial growth on the imprinted material would reduce the adsorption selectivity and shorten the service life. To solve the above problems, carrier materials (GO-PEI/MXene wrinkled microspheres) with dual antibacterial ability are constructed for the first time. BSA surface imprinted GO-PEI/MXene (GO-PEI/MXene@MIPs) are constructed in this study. The large exterior surface area increases the number of imprinting sites, thereby enhancing the adsorption capacity. The adsorption capacity of GO-PEI/MXene@MIPs reaches 312.63 ± 22 mg/g with an imprinting factor (IF) of 3.15 within 90 min. Meanwhile, GO-PEI/MXene@MIPs can separate BSA from mixed protein solution and fetal bovine serum (FBS) and only loses 14.1% of saturation adsorption after eight cycles. In addition, antibacterial tests show that the material has excellent broad-spectrum antibacterial effects, which will significantly extend the service life of the material in real-world environments.