New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)

Zheng Zhang; Xiangmin Yang; Hushan Yang; Xiaoling Yu; Yu Li; Jinliang Xing; Zhinan Chen

doi:10.1016/j.jbiosc.2010.08.012

New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)

Zheng Zhang
, Xiangmin Yang
, Hushan Yang
, Xiaoling Yu
, Yu Li
, Jinliang Xing
, Zhinan Chen

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	111
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiosc.2010.08.012
State	Published - Jan 2011
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

HAb18G/CD147
Protein expression
Site-directed integration system

Access to Document

10.1016/j.jbiosc.2010.08.012

Cite this

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title = "New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)",

abstract = "HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95\% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.",

keywords = "HAb18G/CD147, Protein expression, Site-directed integration system",

author = "Zheng Zhang and Xiangmin Yang and Hushan Yang and Xiaoling Yu and Yu Li and Jinliang Xing and Zhinan Chen",

year = "2011",

month = jan,

doi = "10.1016/j.jbiosc.2010.08.012",

language = "英语",

volume = "111",

pages = "1--6",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)

AU - Zhang, Zheng

AU - Yang, Xiangmin

AU - Yang, Hushan

AU - Yu, Xiaoling

AU - Li, Yu

AU - Xing, Jinliang

AU - Chen, Zhinan

PY - 2011/1

Y1 - 2011/1

N2 - HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.

AB - HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600. mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.

KW - HAb18G/CD147

KW - Protein expression

KW - Site-directed integration system

UR - https://www.scopus.com/pages/publications/78650967062

U2 - 10.1016/j.jbiosc.2010.08.012

DO - 10.1016/j.jbiosc.2010.08.012

M3 - 文章

C2 - 20863755

AN - SCOPUS:78650967062

SN - 1389-1723

VL - 111

SP - 1

EP - 6

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 1

ER -

New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)

Abstract

UN SDGs

Keywords

Access to Document

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