Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy

Yvonne Klapper; Pauline Maffre; Li Shang; Kristina N. Ekdahl; Bo Nilsson; Simon Hettler; Manuel Dries; Dagmar Gerthsen; G. Ulrich Nienhaus

doi:10.1039/c5nr01694k

Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy

Yvonne Klapper
, Pauline Maffre
, Li Shang
, Kristina N. Ekdahl
, Bo Nilsson
, Simon Hettler
, Manuel Dries
, Dagmar Gerthsen
, G. Ulrich Nienhaus

Karlsruhe Institute of Technology
Linnaeus University
Uppsala University
University of Illinois at Urbana-Champaign

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.

Original language	English
Pages (from-to)	9980-9984
Number of pages	5
Journal	Nanoscale
Volume	7
Issue number	22
DOIs	https://doi.org/10.1039/c5nr01694k
State	Published - 14 Jun 2015
Externally published	Yes

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title = "Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy",

abstract = "Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.",

author = "Yvonne Klapper and Pauline Maffre and Li Shang and Ekdahl, \{Kristina N.\} and Bo Nilsson and Simon Hettler and Manuel Dries and Dagmar Gerthsen and Nienhaus, \{G. Ulrich\}",

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doi = "10.1039/c5nr01694k",

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T1 - Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy

AU - Klapper, Yvonne

AU - Maffre, Pauline

AU - Shang, Li

AU - Ekdahl, Kristina N.

AU - Nilsson, Bo

AU - Hettler, Simon

AU - Dries, Manuel

AU - Gerthsen, Dagmar

AU - Nienhaus, G. Ulrich

PY - 2015/6/14

Y1 - 2015/6/14

N2 - Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.

AB - Protein binding to lipid-coated nanoparticles has been pursued quantitatively by using fluorescence correlation spectroscopy. The binding of three important plasma proteins to lipid-enwrapped quantum dots (QDs) shows very low affinity, with an apparent dissociation coefficient in the range of several hundred micromolar. Thus, the tendency to adsorb is orders of magnitude weaker than for QDs coated with dihydrolipoic acid.

UR - https://www.scopus.com/pages/publications/84930323677

U2 - 10.1039/c5nr01694k

DO - 10.1039/c5nr01694k

M3 - 文章

C2 - 25975280

AN - SCOPUS:84930323677

SN - 2040-3364

VL - 7

SP - 9980

EP - 9984

JO - Nanoscale

JF - Nanoscale

IS - 22

ER -

Low affinity binding of plasma proteins to lipid-coated quantum dots as observed by in situ fluorescence correlation spectroscopy

Abstract

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