Fuzzy Mixture-of-Experts Aggregation for Organoid Identification With Multiscale State Space Features

Accurate and automated identification of organoids from bright-field images is essential for enabling high-throughput drug screening and precision medicine. Organoids, as 3-D in vitro cellular models, closely recapitulate the functional and structural characteristics of their tissue or organ of origin, presenting an unprecedented opportunity for biomedical research. However, the complexity of bright-field microscopy images, including heterogeneous backgrounds and diverse organoid morphologies, poses significant challenges for existing computational methods, often hindering robust feature extraction and high-throughput analysis. To address these issues at the intersection of computational vision and organoid biology, we propose FEMSSorg, a novel organoid recognition framework designed to adaptively aggregate multiscale scan-selected state space features through a fuzzy mixture-of-experts (FuzzyMoE) scoring mechanism. FEMSSorg introduces a fuzzy expert soft routing mechanism (fuzzy route), implemented via Fuzzy C-Means-based soft routing assignments, forming a new class of fuzzy MoE that leverages fuzzy expert clustering scores to dynamically integrate local (LocalSS) and global (GlobalSS) state space features. This approach enables effective balancing of global pixel dependencies and local texture information, thereby substantially reducing background interference and image noise in bright-field images and improving the accuracy of organoid identification. Furthermore, we incorporate a Dual Downsampling Adaptive Pooling Feature Fusion module, which combines original backbone features with parallel downsampled features and utilizes content-aware pooling for adaptive multilevel and multiscale feature fusion. Experimental results on multiclass organoid bright-field image datasets demonstrate that FEMSSorg achieves state-of-the-art performance in both organoid detection and morphological texture classification, highlighting its value as a robust computational tool for advancing real-time, high-throughput organoid research.