Fabrication of Metal Ion-BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation

The development of high-performance protein-imprinted materials for adsorption and separation to obtain high-purity, high-quality biomolecules is still one of the most important techniques to promote the rapid development of the protein drug industry. In this paper, a novel highly specific bovine serum albumin (BSA) cobalt-molybdenum double-hydroxides surface-imprinted composite particles (Co-Mo LDHs@MIPs) were constructed by the surface imprinting technique combined with metal chelation. ZIF-67 was etched by molybdate ions to obtain unique MOF-derived bimetallic hydroxide particles (Co-Mo LDHs), which were used as the carrier. The rough outer surface and bimetallic ion coordination of Co-Mo LDHs enable anchoring more template proteins. The adsorption capacity of Co-Mo LDHs@MIPs was up to 423.37 mg/g for BSA in 60 min with an imprinting factor of 2.65. In addition, Co-Mo LDHs@MIPs can selectively recognize and separate BSA from mixed proteins and fetal bovine serum and exhibit high reusability stability.