Extraction and sequencing of trace DNA from Cornu cervi pantotrichum

The trace DNA extraction method was used to extract DNA from the blood, hair, Cornu cervi pantotrichum. hairy antler of the sika deer, Cervus nippon, and penis of Bulbalus bublis and Asinus vulgaris. A 307bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions. The purified PCR products were sequenced by the dideoxy method. DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer, but the sequence of the so - called hairy antler is quite different, implying that it is not from the sika deer. In addition the phylogenetic relationships among different species are congruent with the traditional known phylogeny.