Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles

Liu Deng; Lihua Zhang; Li Shang; Shaojun Guo; Dan Wen; Fuan Wang; Shaojun Dong

doi:10.1016/j.bios.2008.10.031

Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles

Liu Deng, Lihua Zhang, Li Shang, Shaojun Guo, Dan Wen, Fuan Wang, Shaojun Dong

CAS - Changchun Institute of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)₃²⁺ and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)₃²⁺ and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)₃²⁺ film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)₃²⁺ film to promote the electron transfer between Ru(bpy)₃²⁺ and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 μM to 5.2 mM with a detection limit of 12 nM.

Original language	English
Pages (from-to)	2273-2276
Number of pages	4
Journal	Biosensors and Bioelectronics
Volume	24
Issue number	7
DOIs	https://doi.org/10.1016/j.bios.2008.10.031
State	Published - 15 Mar 2009
Externally published	Yes

Keywords

(3-mercaptopropyl)-trimethoxysilane
Alcohol dehydrogenase
Electrochemiluminescence
Gold nanoparticle
NADH
Sol-gel

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10.1016/j.bios.2008.10.031

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title = "Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles",

abstract = "We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)32+ and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)32+ and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)32+ film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)32+ film to promote the electron transfer between Ru(bpy)32+ and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 μM to 5.2 mM with a detection limit of 12 nM.",

keywords = "(3-mercaptopropyl)-trimethoxysilane, Alcohol dehydrogenase, Electrochemiluminescence, Gold nanoparticle, NADH, Sol-gel",

author = "Liu Deng and Lihua Zhang and Li Shang and Shaojun Guo and Dan Wen and Fuan Wang and Shaojun Dong",

year = "2009",

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doi = "10.1016/j.bios.2008.10.031",

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AU - Deng, Liu

AU - Zhang, Lihua

AU - Shang, Li

AU - Guo, Shaojun

AU - Wen, Dan

AU - Wang, Fuan

AU - Dong, Shaojun

PY - 2009/3/15

Y1 - 2009/3/15

N2 - We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)32+ and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)32+ and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)32+ film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)32+ film to promote the electron transfer between Ru(bpy)32+ and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 μM to 5.2 mM with a detection limit of 12 nM.

AB - We developed a stable, sensitive electrochemiluminescence (ECL) biosensor based on the synthesis of a new sol-gel material with the ion-exchange capacity sol-gel to coimmobilize the Ru(bpy)32+ and enzyme. The partial sulfonated (3-mercaptopropyl)-trimethoxysilane sol-gel (PSSG) film acted as both an ion exchanger for the immobilization of Ru(bpy)32+ and a matrix to immobilize gold nanoparticles (AuNPs). The AuNPs/PSSG/Ru(bpy)32+ film modified electrode allowed sensitive the ECL detection of NADH as low as 1 nM. Such an ability of AuNPs/PSSG/Ru(bpy)32+ film to promote the electron transfer between Ru(bpy)32+ and the electrode suggested a new, promising biocompatible platform for the development of dehydrogenase-based ECL biosensors. With alcohol dehydrogenase (ADH) as a model, we then constructed an ethanol biosensor, which had a linear range of 5 μM to 5.2 mM with a detection limit of 12 nM.

KW - (3-mercaptopropyl)-trimethoxysilane

KW - Alcohol dehydrogenase

KW - Electrochemiluminescence

KW - Gold nanoparticle

KW - NADH

KW - Sol-gel

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M3 - 文章

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AN - SCOPUS:60349128336

SN - 0956-5663

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JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 7

ER -

Electrochemiluminescence detection of NADH and ethanol based on partial sulfonation of sol-gel network with gold nanoparticles

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