Effect of osteoblast activity of porous titanium with HA-coating by adding human-like collagen

In this article, osteoblast activity on two functionalised hydroxyapatite (HA) and hydroxyapatite collagen (HA+COL) surface layers is studied, and the effects of the concentrations of collagen and the collagen adding method on osteoblast activity are investigated. The morphology and elements of the HA and HA+COL on porous titanium are observed using a scanning electron microscope and an energy dispersive spectrometer. The number of living osteoblasts is found by comparing the optical density value against a control group. The experimental results showed that osteoblasts have different responses at HA and HA+COL surfaces that have different chemical compositions. The different concentrations and the means of collagen addition can all significantly improve the survival rate of the osteoblasts, but a 7 mg mL^-1 concentration of the added amount of collagen in cell activity gives the best result. It can be concluded that collagen molecules deposit on the HA-coating uniformly and provide a favourable position for the osteoblasts.