@inproceedings{b512747e50004502b4ee5d08521a78b0,
title = "DPCR DUPLEXING METHOD IN A SINGLE FLUORESCENCE CHANNEL",
abstract = "Here, we propose a novel single fluorescence channel-based dPCR duplexing method that combines a specific probe and intercalating dye to increase the difference in fluorescence amplitudes (F) between the two targets. We selected two targets, one detected only by the intercalating dye EvaGreen and the other detected by a combination of a FAM probe and EvaGreen. The results showed the difference in F values between two targets increased from 5% to 32%, when using the FAM probe compared with the detection of both amplicons using EvaGreen only. The added FAM probe enabled two-target discrimination using a single-color fluorescent channel.",
keywords = "Digital PCR, single fluorescence channel duplexing",
author = "Haoqing Zhang and So{\v n}a La{\v s}{\v s}{\'a}kov{\'a} and Zhiqiang Yan and Xinlu Wang and Pavel {\v S}enky{\v r}{\'i}k and Martina Ga{\v n}ov{\'a} and Honglong Chang and Marie Korabecna and Pavel Neuzil",
note = "Publisher Copyright: {\textcopyright} 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",
year = "2022",
language = "英语",
series = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1211--1212",
booktitle = "MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}